HPLC法测定鼻渊片中绿原酸的含量 |
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引用本文: | 张华.HPLC法测定鼻渊片中绿原酸的含量[J].中国药品标准,2010,11(2):127-129. |
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作者姓名: | 张华 |
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作者单位: | 重庆市万州药品检验所,重庆,404000 |
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摘 要: | 目的:建立鼻渊片中绿原酸的含量测定方法。方法:采用HPLC法测定鼻渊片中绿原酸的含量,色谱柱Diamonsil^TM C18(4.6mm×250mm,5μm),流动相为乙腈旬.4%磷酸溶液(8:92);流速为1.0mL·min^-1;检测波长为327nm;柱温为30qC。结果:进样量在0.204—2.040μg范围内绿原酸与峰面积响应值呈良好的线性关系,r=0.9999;平均回收率为100.5%,RSD为0.79%(n=6)。结论:本方法操作简便,结果准确,灵敏度高,重现性好,能有效地控制鼻渊片的质量。
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关 键 词: | 鼻渊片 金银花 绿原酸 HPLC |
Determination of Chlorogenic Acid in Biyuan Tablets by HPLC |
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Authors: | Zhang Hua |
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Institution: | Zhang Hua( Wanzhou Institute for Drug Control of Chongqing, Wanzhou 404000) |
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Abstract: | Objective: To establish a quantitative method for Chlorogenic Acid in Biyuan Tablets by HPLC. Methods: DiamonsilTM C18 column was used. The aeetouitrile-0. 4% phosphoric acid (8: 92) solution was used as the mobile phase. The flow rate was 1.0 mL · min^-1. The detection wavelength was set at 327 nm. Column temperature :30℃. Results: The linear range of Chlorogenic Acid was from 0. 204 -2. 040μg and the correlation coefficient was 0. 999 9. The average recovery rate was 100. 5 % , RSD was 0. 79% (n = 6) Conclusion:The method is convenient and accurate, reproducible and sensitive, which can be used to control the quality of Biyuan Tablets. |
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Keywords: | HPLC |
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