胃癌患者外周血淋巴细胞免疫功能分析

目的分析胃癌患者免疫功能情况，并探讨患者不同临床分期（TNM）与免疫功能之间的关系。方法选择2011年1月～2012年1月胃癌患者62例（病例组）和同期健康成年人62例（对照组），采用流式细胞仪检测外周血淋巴细胞亚群及NK细胞表达水平，并根据国际最新TNM分期标准，比较病例组与对照组、不同临床分期患者免疫功能差异。结果病例组CD3＋、CD4＋、CD4＋／CD8＋、CD16＋CD56＋水平均低于对照组，CD8＋水平高于对照组，差异均有高度统计学意义（均P〈0．01）：Ⅲ、Ⅳ期胃癌患者与Ⅰ、Ⅱ期患者比较，CD4＋／CD8＋、CD16＋CD56＋表达水平较低，而CD8＋水平较高，差异均有高度统计学意义（均P〈0．01）。结论胃癌患者免疫功能较正常人低，临床分期越后，免疫功能越低。检测胃癌患者淋巴细胞亚群水平可有效评价患者免疫功能状态，并对判断患者预后情况具有一定的指导意义。

Analysis of lymphocyte immune function in peripheral blood in patients with gastric cancer

Objective To analyze immune function of the patients with gastric cancer, and to discuss the relationship between different clinical pathological stages （TNM） and immune function. Methods 62 cases of patients with gastric cancer from January 2011 to January 2012 were selected as illness group, 62 cases of healthy adults in the same term were chosen as control group; flow cytometry was used to detect the levels of lymphocytes subsets and the NK cells in peripheral blood, the immune functions were compared between the two groups and different clinical pathological stages according to the new clinical pathological stage （TNM）. Results The levels of CD3＋, CD4＋, CD4＋/CD8＋, CD16＋ CD56＋ in illness group were all lower than those in control group, the differences were all statistically significant （all P 〈 0.01）. The levels of CD4＋/CD8＋, CD16＋CD56＋ in stage Ⅲ,Ⅳ of gastric cancer patients were lower than those in stage Ⅰ, Ⅱ, the levels of CD8＋ in stage Ⅲ, Ⅳ of the patients with gastric cancer was higher than those in stage Ⅰ, Ⅱ, the differences were all statistical significance （all P 〈 0.01）. Conclusion The immune function in the patients with gastric cancer is lower than healthy people and even worse in the lower grade of TNM stage. Detecting the levels of lymphocytes subsets is effective to evaluate the immune function and prognosis of patients with gastric cancer.