支气管哮喘患儿CD4~+CD25~+Foxp3~+IL-10~+ T淋巴细胞水平变化和意义

目的:探讨对尘螨过敏的轻度持续支气管哮喘患儿淋巴细胞增殖情况及CD4+CD25+Foxp3+IL-10+ T淋巴细胞水平变化。方法:选择30例对尘螨过敏的轻度持续支气管哮喘患儿作为实验组,同时选择30例健康儿童作为对照组。分离所有入选者的外周血淋巴细胞,给予户尘螨浸出液刺激48 h后检测淋巴细胞增殖的刺激指数以及CD4+CD25+Foxp3+IL-10+ T淋巴细胞水平变化。结果:支气管哮喘患儿外周血淋巴细胞接受户尘螨浸出液刺激后淋巴细胞增殖的刺激指数显著高于对照组(P<0.01),支气管哮喘患儿CD4+CD25+Foxp3+IL-10+ T淋巴细胞水平显著低于对照组(P<0.01)。结论:支气管哮喘患儿接受过敏原刺激后发生明显的增殖反应,支气管哮喘患儿存在CD4+CD25+Foxp3+IL-10+ T淋巴细胞功能障碍。

Changes and significances of CD4~+CD25~+FOXP3~+IL-10~+ T cells in children with asthma

Objective:To explore the proliferation of T lymphocytes and the changes of CD4+CD25+Foxp3+IL-10+ T lymphocytes in children allergic to house dust mite with mild persistent asthma.Methods:Thirty children allergic to house dust mite(HDM) with mild persistent asthma were selected as experimental group and 30 healthy children were selected as control group.Peripheral blood mononuclear cells(PBMCs) were isolated from the subjects.After 48 hours of in vitro stimulation with HDM extracts,the stimulation indexes(SI) of lymphocytes and the changes of CD4+CD25+Foxp3+ IL-10+ T lymphocytes were measured by flow cytometry.Results:SI of lymphocytes increased significantly in asthma group compared with control group,there was statistically significant difference(P<0.001).The level of CD4+CD25+Foxp3+IL-10+ T lymphocytes in asthma group was statistically significantly lower than that in control group(P<0.01).Conclusion:T lymphocyte proliferation obviously can be observed in asthmatic children when they were stimulated by HDM extract.There is functional insufficiency of CD4+CD25+Foxp3+IL-10+ T lymphocytes in the asthmatic children.