冷冻保存人甲状腺细胞的活力,功能和临床应用

本文研究冷冻保存不同时间甲状腺细胞的活力、功能及其在甲状腺刺激抗体（ＴＳＡｂ）检测中的应用。结果表明：冻存３、６和１２个月的正常或甲亢甲状腺细胞具有良好的活力，各冷冻时相的细胞对ＴＳＨ刺激生成ｃＡＭＰ的反应性无明显差异，且与未冷冻细胞的反应性相似。以冻存甲亢甲状腺细胞测定血清ＴＳＡｂ．在Ｇｒａｖｅｓ病（ＧＤ）未治组的检出率为０．９１３，而甲状腺腺瘤（甲瘤）组与对照组ＴＳＡｂ均为阴性。这提示冻存一年内的人甲状腺细胞仍保持较好的活性与功能，可以作为甲状腺功能和甲状腺疾病基础与临床研究的材料。

The Viability,Function and Clinical Application of Cryopreserved Human Thyroid Cells

The Viability and function of cryopreserved human thyroid cells were investigated and thyroid stimulating antibodies (TSAb) in sera from patients with thyroid disease and controls were measured using cryopreserved hyperfunctioning thyroid cells. The results showed that both the normal and hyperfunctioning thyroid cells cryopreserved for 3,6,or 12 months remained intact and vital. No significant difference in cAMP production was observed among the cells cryopreserved for 3, 6 or 12.months, and the response was similar to noncryopreserved cells. TSAb was found in 21 of 23 patients with untreated Graves' disease, in none of the patients with thyroid adenoma and controls,suggesting that cryopreserved human thyroid cells could be used in the study for the thyroid function and thyroid diseases.