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| 低强度脉冲电磁场对兔动脉粥样硬化斑块中MMP-9及TIMP-1表达的影响 | |
| 引用本文： | 李秀川,陈易华,马双陶,杨大春,罗艳,邱力军. 低强度脉冲电磁场对兔动脉粥样硬化斑块中MMP-9及TIMP-1表达的影响[J]. 西南国防医药, 2011, 21(10): 1064-1067. DOI: 10.3969/j.issn.1004-0188.2011.10.007 |
| 作者姓名： | 李秀川 陈易华 马双陶 杨大春 罗艳 邱力军 |
| 作者单位： | 1. 710032 西安,第四军医大学生物医学工程系;成都军区总医院病理科 2. 成都军区总医院病理科 3. 成都军区总医院心血管内科 4. 第四军医大学生物医学工程系, 西安,710032 |
| 摘 要： | 目的探索低强度脉冲电磁场对大白兔动脉粥样硬化（AS）斑块中基质金属蛋白酶-9（MMP-9）及组织金属蛋白酶抑制物-1（TIMP-1）表达的影响。方法将大白兔随机分成常规饮食（ND）组、高脂饮食（HD）组及高脂饮食＋磁场（HM）组,ND及HD组分别给予常规饮食及高脂饮食,HM组动物在给予高脂饮食的同时,加用低强度脉冲电磁场进行干预。干预7 w后,取主动脉根部行常规石蜡切片及HE染色后,计算AS斑块的面积,采用免疫组化及蛋白免疫印迹法检测AS斑块中MMP-9和TIMP-1的表达。结果高脂饮食可显著增加主动脉根部AS斑块的面积（P〈0.05）,低强度脉冲电磁场干预能显著削弱高脂饮食诱导的AS斑块形成（P〈0.05）;高脂饮食能显著增加AS斑块中MMP-9和TIMP-1的表达（P〈0.05）,而低强度脉冲电磁场干预能显著抑制高脂饮食诱导的MMP-9和TIMP-1表达水平的增加（P〈0.05）,且可显著降低MMP-9与TIMP-1的比值。结论低强度脉冲电磁场抗AS的作用机制可能与降低MMP-9与TIMP-1的比值有关。 |
| 关 键 词： | 动脉粥样硬化 低强度脉冲电磁场 MMP-9 TIMP-1 |
Effects of low-intensity pulse electromagnetic field on expression of MMP-9 and TIMP-1 in atherosclerotic plaques of rabbits |
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| Li Xiuchuan,Chen Yihua,Ma Shuangtao,Yang Dachun,Luo Yan,Yang Yongjian,Li De,Shen Guanghao,Luo Erping. Effects of low-intensity pulse electromagnetic field on expression of MMP-9 and TIMP-1 in atherosclerotic plaques of rabbits[J]. Medical Journal of National Defending forces in Southwest China, 2011, 21(10): 1064-1067. DOI: 10.3969/j.issn.1004-0188.2011.10.007 | |
| Authors: | Li Xiuchuan Chen Yihua Ma Shuangtao Yang Dachun Luo Yan Yang Yongjian Li De Shen Guanghao Luo Erping |
| Affiliation: | Li Xiuchuan1,Chen Yihua2,Ma Shuangtao3,Yang Dachun3,Luo Yan2,Yang Yongjian3,Li De3,Shen Guanghao1,Luo Erping11.Department of Biomedical Engineering,Fourth Military Medical University,Xi'an,Shanxi,710032,China,2.Department of Pathology,3.Department of Cardiology,General Hospital of Chengdu Military Command,Chengdu,Sichuan,610083 |
| Abstract: | Objective To investigate the effects of low-intensity pulse electromagnetic field(LPEF)on the expression of matrix metalloproteinase-9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)in atherosclerotic(AS)plaques of rabbits.Methods Rabbits were randomly divided into three groups:normal diet(ND)group,high fat diet(HD)group and HD plus magnetic field(HM)group.The ND and the HD group were respectively given regular diet and high fat diet.The HM group was given high fat diet,and meanwhile received inte... |
| Keywords: | atherosclerosis low-intensity pulse electromagnetic field MMP-9 TIMP-1 |
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