联合应用抗菌药物对耐药鲍曼不动杆菌体外抗菌活性的研究

目的了解抗菌药物对多重耐药鲍曼不动杆菌的体外联合抗菌活性，为临床治疗多重耐药鲍曼不动杆菌感染提供实验依据。方法采用常规培养方法分离细菌，应用全自动细菌分析仪对其进行鉴定。药敏试验采用K—B纸片法，MIC测定采用琼脂平板倍比稀释法，按CLSI规定标准进行。结果从临床分离的菌株中共筛选出30株多重耐药鲍曼不动杆菌。奈替米星对多重鲍曼不动杆菌没有抑菌作用；亚胺培南、美罗培南的敏感率约为50％；其他常用抗菌药物（如头孢哌N／舒巴坦、多西环素、利福平、莫西沙星）的敏感率均小于35％。碳青酶烯类药物（亚胺培南、美罗培南）与头孢哌酮／舒巴坦联合的协同作用高于其他抗菌药联合物；多西环素、利福平分别与亚胺培南、美罗培南联合时均无协同作用；所有这些联合抗菌药物均未出现拮抗作用。结论对多重耐药鲍曼不动杆菌引起的感染，应选择亚胺培南或美罗培南与头孢哌酮／舒巴坦联合治疗，以有效地控制感染，防止耐药株在医院内感染的扩散。

Antibacterial activity of combined antibacterials against drug resistant Acinetobacter baumannii in vitro

Objective To study the antibacterial activity of combined antibacterials against multidrug resistant Acinetobacter baumannii in vitro,and provides experimental data for clinical treatmen.Methods All the strains were separated by the method of conventional culture and identified by automatic bacterial analysator.Kirby-Bauer paper disk method was used in the susceptibility test.Agar plate double dilution was used in the MIC detection according to the standard of CLSI.Results Thirty strains of multidrug-resist...