Identification and Partial Purification of lnterferons from Human FirstTrimester Trophoblast Tissue

Previous researeh demonstrated the presence of interferons with unusual moleeularweights and amigenicity in virus-infected amniotie membrane and in placental bloodat mid-term and term pregnaney. We reported here the identificution and partial purification of interferons from aeid extract of humen first trimester trophoblast tissue,Immunoreactive interferons, inchuling interferon-x and -β were detected with immunoelectrophoresis and enzyme-linked immunosorbent assays, By preparative is oelectrofocusing, preparative polyacrylamide get elcurophoresis and electroelution,one relatively pure protein with molecular weight of 43 kilodalton was found to have interferon x/β immunoreactivhy,the physiolo gical role(s)of these interferons in the maintenance of pregnancy remains to be defined.