| 高效液相色谱法测定星虫体壁胶原蛋白 |
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| 引用本文： | 孙蓓,苏秀榕,林叶,张威.高效液相色谱法测定星虫体壁胶原蛋白[J].中国生化药物杂志,2007,28(6):404-406. |
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| 作者姓名： | 孙蓓 苏秀榕 林叶 张威 |
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| 作者单位： | 宁波大学生命科学与生物工程学院,浙江,宁波315211 |
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| 摘 要： | 目的建立快速检测胶原蛋白的高效液相色谱法。方法采用异硫氰酸苯酯柱前衍生反相高效液相色谱,以乙晴-醋酸盐缓冲系统梯度洗脱,于254 nm波长处根据羟脯氨酸的含量来推算星虫体壁胶原蛋白。结果羟脯氨酸衍生物与其它氨基酸较好分离,在5~200μg/mL浓度范围内有良好的线性关系(r=0.999 9),回收率为102.6%,RSD为1.58%。结论此法快速、灵敏、分辨率高,适用于动物组织中胶原蛋白含量的测定。
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| 关 键 词： | 星虫 胶原蛋白 羟脯氨酸 异硫氰酸苯酯 高效液相色谱法 |
| 文章编号： | 1005-1678(2007)06-0404-02 |
| 修稿时间： | 2007年7月2日 |
Determination of parietal collagen in Sipunculid by high performance liquid chromatography |
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| SUN Bei,SU Xiu-rong,LIN Ye,ZHANG Wei.Determination of parietal collagen in Sipunculid by high performance liquid chromatography[J].Chinese Journal of Biochemical Pharmaceutics,2007,28(6):404-406. |
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| Authors: | SUN Bei SU Xiu-rong LIN Ye ZHANG Wei |
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| Abstract: | Purpose To set up a method of HPLC to determine the content of parietal collagen in Sipunculid.Methods RP-HPLC was used by precolumn derivation with phenylisothiocyanate.Acetonitrile-acetate buffer linear gradient elution was used.Detecting wavelength was 254 nm.Results Derivation of hydroxyproline could be separated from other amino acids.Hydroxyproline showed good linear(r=0.999 9) in the range of 5～200 μg/mL.The recovery was 100.1%～104.6%.RSD was 1.58%.Conclusion The method is rapid,and accurate,and it is suitable for detection of collagen samples obtained from animal tissues. |
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| Keywords: | Sipunculid collagen hydroxyproline phenylisothiocyanate HPLC |
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