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| 恶性胶质瘤细胞在不同培养液和不同时期中CD133阳性细胞比率的变化 | |
| 引用本文： | 花玮,周良辅,毛颖,肖保国,朱剑宏,王晓梅,姚瑜.恶性胶质瘤细胞在不同培养液和不同时期中CD133阳性细胞比率的变化[J].中国临床神经科学,2008,16(6):565-571. |
| 作者姓名： | 花玮 周良辅 毛颖 肖保国 朱剑宏 王晓梅 姚瑜 |
| 作者单位： | 1. 复旦大学附属华山医院神经外科,200040 2. 复旦大学附属华山医院神经外科,200040;复旦大学神经病研究所,200040 3. 复旦大学神经病研究所,200040 |
| 基金项目： | 国家自然科学基金 |
| 摘 要： | 目的:观察不同培养液及不同培养时期等因素对体外培养人脑胶质瘤细胞CD133阳性(CD133+)细胞比率的影响.方法:用干细胞培养液(含EGF、FGF-2、B27)培养6例原代胶质瘤细胞和3例对照细胞株,用流式细胞仪检测培养第0、3、7,28、60、90和120天时不同细胞的CD133+细胞比率;免疫组化观察胶质瘤干细胞的多向分化能力;用血清、无血清和干细胞3种培养液分别培养2例长期培养的胶质瘤SHG62、SH066细胞系以及对照的U87和U251细胞株,观察胶质瘤中CD133+细胞在不同培养液中的相互转换.结果:胶质瘤CD133+细胞比率随培养时间推移明显下降(P＜0.05),1周左右均降至＜1%的低水平,而细胞株则比较稳定;干细胞培养液培养的细胞中CD133+细胞比率明显高于血清和无血清培养液培养(P＜0.01);此外,胶质瘤细胞中的干细胞具有多向分化的能力,并且可以在干细胞和血清培养液中互相转换.结论:原代胶质瘤体外培养过程中CD133+细胞比率逐渐下降,而干细胞培养液能明显提高CD133+细胞比率,并且CD133+细胞可以在不同培养液中相互转换.说明生长环境对于细胞比率影响较大,提示可能存在尚未明了的影响干细胞生长分化的相关因子. |
| 关 键 词： | 恶性胶质瘤 干细胞 |
Ratio of CD133 Positive Expressed Cells in Malignant Glioma in Different Mediums and Culture Phases in Vitro |
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| HUA Wei,ZHOU Liang-Fu,MAO Ying,XIAO Bao-Guo,ZHU Jian-Hong,WANG Xiao-Mei,YAO Yu.Ratio of CD133 Positive Expressed Cells in Malignant Glioma in Different Mediums and Culture Phases in Vitro[J].Chinese Journal of Clinical Neurosciences,2008,16(6):565-571. | |
| Authors: | HUA Wei ZHOU Liang-Fu MAO Ying XIAO Bao-Guo ZHU Jian-Hong WANG Xiao-Mei YAO Yu |
| Institution: | HUA Wei~1 ZHOU Liang-Fu~(1,2) MAO Ying~1 XIAO Bao-Guo~2 ZHU Jian-Hong~1 WANG Xiao-Mei~1 YAO Yu~1 ~1Department of Neurosurgery,Huashan Hospital,Fudan University,Shanghai,200040,~2Institute of Neurology,Shanghai 200040,China |
| Abstract: | Aim:To find the dynamic changes of CD133~+(CD133 positively expressed)cells in primary culture and also in different mediums.Methods:Six primary malignant glioma cells and 3 glioma cell strains were cultured in stem cell medium(supplemented with EGF,FGF-2 and B27),then their ratios of CD133~+ cells were checked with flowcytometry on day 0,3,7,28,60,90,120 respectively.Multiple differentiations of stem cells were proved by immunocytochemistry.Then changes of the ratios of CD133~+ cells of SHG62,SHG66 (long-t... |
| Keywords: | CD133 |
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