S100A4调节MMP-13表达对甲状腺癌细胞侵袭能力的影响

目的探讨靶向S100A4对甲状腺癌细胞侵袭和转移的影响。方法以甲状腺癌BCPAP细胞或暴露于MMP-13抗体24h后的BCPAP细胞为研究对象,将S100A4基因小干扰RNA(siRNA)或S100A4/cDNA通过脂质体转染法转染BCPAP细胞;48h后采用RT-PCR和蛋白质印迹法检测S100A4mRNA、MMP-13mRNA和蛋白水平;Transwell侵袭实验和划痕实验检测BCPAP细胞侵袭能力的改变。结果转染S100A4siRNA 48h后,siRNA组S100A4mRNA表达量为(0.25,0.12),阴性对照组(NC)为(1.19,0.41),空白对照(Blank)组为(1.22,0.38),差异有统计学意义,H=13.68,P<0.05;siRNA组S100A4蛋白表达量为(0.40,0.21),NC组为(0.88,0.29),Blank组为(0.91,0.36),差异有统计学意义,H=15.74,P<0.05;siRNA组MMP-13mRNA表达量为(0.23,0.13),NC组为(1.08,0.28),Blank组为(1.12,0.37),差异有统计学意义,H=14.92,P<0.05;siRNA组MMP-13蛋白表达量为(0.42,0.16),NC组为(1.03,0.35),Blank组为(1.07,0.41),差异有统计学意义,H=17.64,P<0.05。质粒瞬时转染48h后,S100A4cDNA组S100A4蛋白表达量为(0.96,0.34),pCMV2-Myc组为(0.35,0.19),Blank组(0.37,0.22),差异有统计学意义,H=19.76,P<0.05。S100A4cDNA组MMP-13蛋白表达量为(1.21,0.47),pCMV2-Myc组为(0.38,0.21),Blank组为(0.37,0.23),差异有统计学意义,H=21.53,P<0.05;anti-MMP-13组蛋白表达量为(0.25,0.14),与S100A4cDNA组相比差异有统计学意义,z=5.89,P<0.05。细胞转染siRNA 48h后,Blank组穿膜细胞数为(37.86,3.52),NC组为(38.59,4.74),siRNA组为(11.38,2.16),差异有统计学意义,H=16.28,P<0.05;Blank组划痕48h后宽度为(0.24,0.11)cm,NC组为(0.28,0.05)cm,siRNA组为(1.98,0.13)cm,差异有统计学意义,H=16.65,P<0.05。质粒瞬时转染细胞48h后,Blank组穿膜细胞数为(39.68,3.76),pCMV2-Myc组为(41.66,8.02),S100A4cDNA组为(70.14,7.85),差异有统计学意义,H=19.24,P<0.05;anti-MMP-13组为(43.56,7.86),与S100A4cDNA组相比差异有统计学意义,z=5.87,P<0.05;Blank组划痕48h后宽度为(0.31,0.11)cm,pCMV2-Myc组为(0.29,0.09)cm,S100A4cDNA组为0,差异有统计学意义,H=21.84,P<0.05;anti-MMP-13组为(1.96,0.17)cm,与S100A4cDNA组相比差异有统计学意义,z=12.03,P<0.05。结论S100A4通过调节MMP-13表达影响甲状腺癌细胞的侵袭能力,靶向S100A4可下调MMP-13表达抑制甲状腺癌的侵袭转移。

Effect of S100A4 on invasion of human thyroid carcinoma cells by regulation of MMP-13