2型糖尿病并脑梗死患者eNOS基因内含子4上a/b基因多态性的初步研究

目的探讨内皮细胞性一氧化氮合酶(eNOS)基因内含子4上a/b基因的多态性与单纯2型糖尿病(NIDDM)及糖尿病并脑梗死(cerebral infarction,CI)的关系.方法应用聚合酶链反应(PCR)技术,检测89例NIDDM合并CI病人的基因型,并与对照组比较.结果 NIDDM合并CI组eNOS基因ab基因型的频率(26.97%)明显高于对照组(12.5%)及NIDDM组(12.68%),a等位基因的频率(13.48%)也明显高于对照组(6.25%)及NIDDM组(6.34%),均有显著性差异(P＜0.05).结论 eNOS基因ab基因型与NIDDM合并CI有相关性,等位基因a可能是NIDDM合并CI的危险因素.

Research about the a/b in intron 4 polymorphism of endothelial nitric oxide synthase (Enos)gene in type 2 diabetes with cerebral infarction

Objective To investigate association between the a/b in intron 4 polymorphism of endothelial nitric oxide synthase(eNOS) gene and type 2 diabetes with cerebral infarction(CI).Methods The genotype of 89 patients with type 2 disbetes with Cl and control group and type 2 diabetes group was measured by polymerase chain reaction(PCR).Results The ab genotype distibution frequency of eNOS gene in diabetes group with CI is higher than that in control group(26.97%vs12.5%)and type 2 diabetes(12.68%).The a allele frequency of eNOS gene of diabetes group with CI is significantly higher than that in control group(13.48%vs6.25%),and significantly higher than that in type 2 diabetes(6.34%),too.Statistical differences between diabetes group with CI and control group are significant(P<0.05),and between diabetes group with CI and diabetes group(P<0.05).Conclusion There is association between ab genotype of eNOS and diabetes with CI.The eNOS a allele may be a risk factor for diabetes with CI.