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锌离子螯合剂对脂多糖诱导的小胶质细胞中细胞因子表达的影响
引用本文:陆颖,周松林,赵健亚,徐广飞. 锌离子螯合剂对脂多糖诱导的小胶质细胞中细胞因子表达的影响[J]. 中华老年心脑血管病杂志, 2012, 14(10): 1093-1095
作者姓名:陆颖  周松林  赵健亚  徐广飞
作者单位:南通大学公共卫生学院营养与食品卫生学教研室,226019
摘    要:目的研究锌离子螯合剂四吡啶甲基乙二胺(TPEN)对脂多糖(LPS)诱导的小胶质细胞中细胞因子表达的影响及机制。方法体外培养小鼠小胶质瘤细胞系小胶质细胞后分为对照组、LPS组(100 ng/ml LPS,30 min或4h)、TPEN+LPS组(25μmol/L TPEN,1 h+LPS,30 min或4 h)、细胞外信号调节激酶(ERK)抑制剂PD98059+LPS组(PD98059+LPS组,25μmol/L PD98059,30 min+LPS,4 h),采用实时定量PCR法分析细胞因子白细胞介素(IL)-1β、IL-6、TNF-αmRNA的表达,Western blot法检测pERK1/2蛋白的表达。结果与对照组比较,LPS组pERK1/2蛋白表达和IL-1β、IL-6、TNF-αmRNA表达明显升高(P<0.05,P<0.01);与LPS组比较,TPEN+LPS组pERK1/2蛋白表达和IL-1β、IL-6、TNF-αmRNA表达明显降低(P<0.05,P<0.01),而PD98059+LPS组IL-1β、IL一6、TNF-αmRNA表达明显降低(P<0.01)。结论 TPEN可能通过减少pERK1/2表达来抑制LPS诱导的细胞因子的大量释放。

关 键 词:脂多糖类  小神经胶质细胞  细胞因子类  锌化合物  螯合剂  细胞外信号调节MAP激酶类

Effect of zinc chelator on lipopolysaccharide-induced cytokine expression in microglial cells
Affiliation:LU Ying,ZHOU Song-lin,ZHAO Jian-ya,et al (Teaching and Research Section of Nutrion and Food Hygiene,Public Health School,Nantong University,Nantong 226019,Jiangsu Province,China)
Abstract:Objective To study the effect of zinc chelator(TPEN) on lipopolysaccharide(LPS)-induced cytokine expression in microglial cells.Methods In vitro cultured mouse microglial cells were divided into normal control group,LPS group(treated with 100 ng/ml LPS for 30 min or 4 h),TPEN + LPS group(treated with 25μmol/L TPEN for 30 min or 4 h and+LPS for 1 h),and ERK inhibitor PD98059 + LPS group(treated with 25μmol/L PD98059 for 4 h and + LPS for 30 min).Expressions of IL-1β,IL-6 and TNF-αmRNA were detected by RT-PCR.Expression of pERK1/2 protein was detected by Western blot.Results The expression levels of pERK1/2 protein and IL-1β,IL-6 and TNF-αmRNA were significantly higher in LPS group than in normal control group(P<0.05,P<0.01) and significantly lower in TPEN + LPS group than in LPS group (P<0.05,P<0.01).The expression levels of IL-1β,IL-6 and TNF-αmRNA were significantly lower in PD98059 + LPS group than in LPS group(P<0.01).Conclusion TPEN inhibits LPS-induced massive release of cytokines possibly by down-regulating the expression of pERKl/2.
Keywords:lipopolysaccharides  microglia  cytokines  zinc compounds  chelating agents  extracellular signal-regulated MAP kinases
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