囊性纤维化跨膜转导调节因子在子宫内膜中表达的变化及其意义

目的　探讨囊性纤维化跨膜转导调节因子 (CFTR)在人月经周期子宫内膜中表达的变化及其意义。方法　采用免疫组化、免疫印迹、原位杂交方法检测人月经周期子宫内膜中CFTR的蛋白及mRNA的分布与含量变化。结果　 (1)CFTR仅在子宫内膜腺上皮细胞中表达 ,增生期晚期、分泌期、月经期内膜腺上皮细胞中均富含CFTR ,与增生期早期相比 ,差异有显著性 (P <0 0 5 ) ;免疫印迹法证实 ,增生期晚期、分泌期内膜存在特异性的、相对分子质量为 170 0 0 0的CFTR条带 ;(2 )CFTRmRNA在增生期晚期和分泌期早期子宫内膜中含量最高 ,与增生期早期、分泌期晚期相比 ,差异有显著性 (P<0 0 5 )。结论　人月经周期子宫内膜腺上皮细胞中存在CFTRmRNA和蛋白的表达 ,含量均随月经周期而变化 ;增生期晚期CFTR表达的增强。分泌期CFTR的表达及局部调节可能参与人类胚泡着床的调控。

Expression of cystic fibrosis transmembrane conductance regulator in human endometrium

Objective To study expression of cystic fibrosis transmembrane conductance regulator (CFTR) in human endometrium. Methods The expression of CFTR mRNA and protein from 50 samples of normal cyclic human endometrium was examined by in situ hybridization, immunohistochemistry and Western blotting respectively. Results CFTR mRNA and protein expressions were only detected in the endometrial glandular cells with cyclic changes. CFTR mRNA could be detected from the midproliferative phase with the highest level found in the late proliferative phase, significantly higher than those of late-secretory phase endometrium ( P <0.05). While a large quantity of CFTR protein were seen in late proliferative phase and still presented in the secretive and menstrual phases. Western blotting analysis demonstrated that human endometrium expressed the special CFTR band at 170 000. Conclusion The expression of CFTR in human endometrium presented in glandular cells and the amounts were varied cyclically. The abundant CFTR mRNAs and proteins around the ovulatory period may drive Na and fluid from plasma into the uterine lumen to make optimal electrolyte composition and sufficient fluid volume for sperm migration.