原位杂交检测骨巨细胞瘤u-PA、u-PAR和PAI-2 mRNA的表达及意义

目的 研究骨巨细胞瘤（giant cell tumors of bone,GCT)组织u-PA、u-PAR和PAI－2mRNA的表达与GCT病理分级和复发的关系。方法 用原位杂交技术检测42例GCT组织u-PA和u-PAR和PAI－2 mRNA的表达。结果 （1）u-PA、u-PAR和PAI－2mRNA在GCT组织多核巨细胞（MGC）的阳性率分别为64．3％、71．4％和40．5％；单核基质细胞（MC）的阳性率分别为54．8％、45．2％和9．5％。（2）Ⅱ、Ⅲ级和复发组GCT的MGC u-PA mRNA与MC u-PA、u-PAR mRNA的表达率均明显高于Ⅰ级和无复发组（P均＜0．05）。（3）GCT组织两种细胞间的u-PA mRNA表达、MGC u-PAR与PAI－2 mRNA的表达间、MCu-PA与u-PAR mRNA的表达间均呈正相关；MGC PAI－2mRNA的阳性率明显高于MC的阳性率（P均＜0．05）。结论 u-PA、u-PAR mRNA在GCT组织中的表达，尤其是在MC中的表达，提示肿瘤分化较低和有复发倾向；MC可能是GCT中主要的肿瘤细胞。

Expression and significance of urokinase-type plasminogen activator, its receptor, and type 2 inhibitor in giant cell tumor of bone

OBJECTIVE: To investigate the expression and significance of u-PA, u-PAR and PAI-2 mRNA in giant cell tumor of bone. METHODS: The expressions of u-PA, u-PAR and PAI-2 mRNA in 42 patients with giant cell tumor(GCT) of bone was detected with in situ hybridization. RESULTS: The expression rates of u-PA, u-PAR, and PAI-2 mRNA of multinuclear giant cells (MGC) were 64.3%, 71.4% and 40.5%. Their expression rates in the mononuclear cells (MC) were 54.8%, 45.2% and 9.5%, respectively. The expression rates of u-PA mRNA in MGC, u-PA and u-PAR mRNA in MC of grade II and III lesions were significantly higher than those of grade I and non-recurrent lesions (P < 0.05). Close positive relation was observed between the expressions of u-PA mRNA of both cells, between the expressions of u-PAR and PAI-2 mRNA in MGC and also between the expressions of u-PA and u-PAR mRNA in MC. The expression rate in MGC was higher than that of MC significantly (P < 0.05). CONCLUSION: The expressions of u-PA, u-PAR mRNA of giant cell tumor, especially in the mononuclear cells demonstrate lower differentiation and recurrent potential in giant cell tumor of bone. Mononuclear cells may be the main tumor cell in giant cell tumor of bone.