E2F-1和Rb基因表达与乳腺乳头状瘤病及导管内癌的相关性

目的　研究细胞周期调控因子E2F 1和Rb与乳腺乳头状瘤病及导管内癌的相关性 ,探讨乳头状瘤病癌变的某些分子机制。方法　采用原位杂交法和免疫组化法对 4 0例乳腺轻度乳头状瘤病、4 0例重度乳头状瘤病和 4 0例导管内癌进行了检测 ,观察E2F 1和Rb基因mRNA和蛋白的表达情况。结果　E2F 1mRNA表达阳性率在乳腺轻度乳头状瘤病、重度乳头状瘤病和导管内癌中分别为17.5 %、4 5 .0 %和 80 .0 % ;蛋白表达阳性率分别为 2 0 .0 %、4 7.5 %和 77.5 %。E2F 1mRNA和蛋白表达三组间差异有显著性 (P <0 .0 1) ,组间两两比较 ,差异也有显著性 (P <0 .0 1)。Rb基因mRNA表达阳性率在乳腺轻度乳头状瘤病、重度乳头状瘤病和导管内癌中分别为 90 .0 %、5 0 .0 %和 2 0 .0 % ;蛋白表达阳性率分别为 85 .0 %、5 2 .5 %和 2 2 .5 %。Rb基因mRNA和蛋白表达三组间差异有显著性 (P <0 .0 1) ,组间两两比较 ,差异也有显著性 (P <0 .0 1)。随着乳腺乳头状瘤病程度的加重最终进展为癌 ,E2F 1mRNA和蛋白表达阳性率呈上升趋势 ;RbmRNA和蛋白表达阳性率呈下降趋势。E2F 1和RbmRNA表达均与蛋白表达呈正相关 ,但这两种调控因子的表达呈负相关。结论　E2F 1和Rb基因mRNA和蛋白表达可作为癌症早期较有价值的参考指标 ,有助于从乳腺乳头状瘤病

Correlation of E2F-1 and Rb expression with papillomatosis and ductal carcinoma in situ of the breast

Objective E2F-1 and Rb are involved in cell cycle regulation. This study was to illustrate the mechanism of transformation from benign papillomatosis to ductal carcinoma in situ (DCIS) of the breast in relation to E2F-1 and Rb expression. Methods In situ hybridization (ISH) was used to determinate the expression of E2F-1 and Rb mRNA of mild papillomatosis (MP, n=40), severe papillomatosis (SP, n=40) and DCIS (n=40). Immunohistochemistry (IHC) was used to examine the expression of E2F-1 and Rb protein. Results The positive rate of E2F-1 mRNA expression in MP, SP and DCIS was 17.5%, 45.0% and 80.0%, and that of E2F-1 protein expression was 20.0%, 47.5% and 77.5%, respectively. There were significant differences among the three groups (P<0.01), and between any two groups (P<0.01). The positive rate of Rb mRNA expression in MP, SP and DCIS was 90.0%,50.5% and 20.0%, and that of Rb protein expression was 85.0%, 52.5% and 22.5%, respectively, with statistically significant difference similar with that of E2F-1. With the progression of papillomatosis to DCIS, the expression of E2F-1 mRNA and protein increased, while that of Rb decreased. The protein expression by IHC was positively correlated with the mRNA expression by ISH. However, that of E2F-1 was negatively correlated with Rb. Conclusion E2F-1 and Rb might provide a valuable basis for screening high risk papillomatosis and new target of gene therapy for pre-cancerous lesions of the breast.