| 经鼻给予降钙素基因相关肽促进蛛网膜下腔出血后大脑皮层CD31/PECAM-1表达 |
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| 引用本文： | 杨明峰,申发平,袁慧,谢方民,张颜波,孙保亮.经鼻给予降钙素基因相关肽促进蛛网膜下腔出血后大脑皮层CD31/PECAM-1表达[J].泰山医学院学报,2009,30(12). |
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| 作者姓名： | 杨明峰 申发平 袁慧 谢方民 张颜波 孙保亮 |
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| 作者单位： | 1. 山东省高校脑微循环重点实验室、泰山医学院附属医院,山东泰安,271000
2. 山东省高校脑微循环重点实验室、泰山医学院附属医院,山东泰安271000;大庆油田总医院集团龙南医院,黑龙江大庆163001 |
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| 基金项目： | 国家自然科学基金，山东省自然科学基金，山东省教育厅科技计划，山东省中医药科技发展计划，山东省卫生系统高层次人才1020工程基金 |
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| 摘 要： | 目的探讨经鼻应用降钙素基因相关肽(CGRP)对蛛网膜下腔出血(SAH)后大脑皮层血小板内皮细胞粘附分子-1(CD31/PECAM-1)表达的影响。方法将SPF级雄性Wistar大鼠随机分入正常对照组、SAH组、经鼻生理盐水(NS)+SAH、经鼻CGRP+SAH组。用枕大池两次注入自体动脉血法制作SAH模型,CGRP和NS经鼻腔给予。于第二次枕大池注血后3d,将大鼠经心灌注生理盐水后取脑制作冰冻切片,用免疫荧光结合激光共聚焦显微镜检测大脑皮层CD31/PECAM-1表达。结果行为学和解剖学观察证实大鼠SAH模型制作成功。第二次枕大池注血后3d,SAH组和经鼻NS+SAH组大鼠大脑皮层CD31/PECAM-1表达增多,经鼻CGRP+SAH组大鼠大脑皮层CD31/PECAM-1表达进一步增强。结论经鼻应用CGRP可促进SAH后大脑皮层CD31/PECAM-1表达,从而在一定程度上促进血管新生,减轻继发性脑缺血。
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| 关 键 词： | 蛛网膜下腔出血 继发性脑缺血 血小板内皮细胞粘附分子-1 血管新生 |
Increased expression of CD31/PECAM-1 in cerebral cortex by intranasal delivery of calcitonin gene-related peptide after subarachnoid hemorrhage |
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| YANG Ming-feng,SHENG Fa-ping,;,YUAN Hui,XIE Fang-Min,ZHANG Yan-bo,SUN Baoliang.Increased expression of CD31/PECAM-1 in cerebral cortex by intranasal delivery of calcitonin gene-related peptide after subarachnoid hemorrhage[J].Journal of Taishan Medical College,2009,30(12). |
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| Authors: | YANG Ming-feng SHENG Fa-ping ; YUAN Hui XIE Fang-Min ZHANG Yan-bo SUN Baoliang |
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| Institution: | 1(1.Key Lab of Cerebral Microcirculation in Universities of Shandong; Affiliated Hospital of TaishanMedical College; Taian 271000; China; 2.Longnan Hospital; Daqing Oil Field General Hospital Group; Daqing 163001; China); |
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| Abstract: | Objective:To investigate the effects of intranasal delivery of calcitonin gene-related peptide(CGRP) on the expression of CD31/ PECAM-1 in cerebral coetex following experimental subarachnoid hemorrhage(SAH).Methods: SPF level Male Wistar rats were randomly divided into normal control group,SAH group,intranasal normal saline(NS) +SAH group and intranasal CGRP+SAH group.SAH models were produced by double injection of autologous arterial blood into cisterna magna.CGRP and NS were given by intranasal perfusion.... |
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| Keywords: | subarachnoid hemorrhage secondary cerebral ischemia CD31/ PECAM-1 angiogenesis |
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