| EFFECT OF PLATELET HEPARIN AFFINITY MATERIALS ON ENDOTHELIAL CELL FIBRINOLYTIC ACTIVITY AND SECRETION OF PROSTACYCLIN |
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| 引用本文： | 吴,任文华,陈岚,徐也鲁. EFFECT OF PLATELET HEPARIN AFFINITY MATERIALS ON ENDOTHELIAL CELL FIBRINOLYTIC ACTIVITY AND SECRETION OF PROSTACYCLIN[J]. 上海第二医科大学学报, 1993, 0(1) |
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| 作者姓名： | 吴 任文华 陈岚 徐也鲁 |
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| 作者单位： | Department of Pathophysiology，Shanghai Institute of Hematology，SSMU，Department of Pathophysiology，Shanghai Institute of Hematology，SSMU，Department of Pathophysiology，Shanghai Institute of Hematology，SSMU，Department of Pathophysiology，Shanghai Institute of Hematology，SSMU Shanghai，Shanghai，Shanghai，Shanghai |
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| 摘 要： | Platelets and endothelial cells play an important role in initiation anddevelopment of atherosclerosis. Much attention has been paid to investigate effect ofplatelet release products on endothelial cells, it is unknown whether platelet products canaffect endothelial cell fibrinolytic activity. We extracted platelet heparin affinity materials(PHAM) from platelet lysate using Heparin-Sepharose CL 6B column, and treated cul-tured human umbilical endothelial cells with PHAM to imitate conditions in vivo. As a re-suit, PHAM could increase endothelial cell PAI activity without change of t-PAantigen. No free t-PA activity was detected in conditioned medium from either stimulatedor nonstimulated endothelial cell cultures. Addition of 10μg/ml PHAM to endothelialcells induced nearly one fold increase of PAI activity (P<0.001). Such increase was timeand dose dependent. Moreover, treatment with PHAM resulted in a dose dependent in-crease of the secretion of prostacyclin (measured by 6-keto-PGF_(1α)), 10μg/ml PHAMcaused six fold increase of prostacyclin (P<0.001). The above results showed thatPHAM could inhibit endothelial cell fibrinolytic activity but increase secretion ofprostacyclin. It is assumed that this could be the negative feedback for protecting thehuman body from thrombus formation. Break of this feedback mechanism may beresponsible for thrombotic diseases.
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EFFECT OF PLATELET HEPARIN AFFINITY MATERIALS ON ENDOTHELIAL CELL FIBRINOLYTIC ACTIVITY AND SECRETION OF PROSTACYCLIN |
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| Wu Yan,Ren Wenhua,Chen Lan,Xu Yelu. EFFECT OF PLATELET HEPARIN AFFINITY MATERIALS ON ENDOTHELIAL CELL FIBRINOLYTIC ACTIVITY AND SECRETION OF PROSTACYCLIN[J]. Journal of Shanghai Second Medical University(Foreign Language Edition), 1993, 0(1) |
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| Authors: | Wu Yan Ren Wenhua Chen Lan Xu Yelu |
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| Affiliation: | Wu Yan,Ren Wenhua,Chen Lan,Xu Yelu Department of Pathophysiology,Shanghai Institute of Hematology,SSMU,Shanghai |
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| Abstract: | |
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| Keywords: | endothelial cell platelet atherosclerosis platelet heparin affinity materials |
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