芹菜素人体内代谢研究

目的 研究摄入芹菜叶后芹菜素在人体内的吸收、排泄。方法 10名健康受试者,男女各半,年龄范围24~28岁,平均体质指数(BMI)为20.37 kg/m²。受试者按2g/(kg.bw)食用芹菜叶并食用不含芹菜素和毛地黄黄酮的食物。在分别摄入芹菜叶后4,6,7,8,9,10,11和28 h,采集血样,并收集24 h全尿样。用高效液相色谱-电化学检测器(HPLC-ECD)检测血浆、红细胞和尿中芹菜素含量。结果 摄入芹菜叶后,全部受试者血浆中芹菜素浓度呈上升趋势,(7.70±0.82)h血浆芹菜素浓度达最高水平(207.11±97.55)nmol/L,在28 h内下降到检出限(2.32 nmol/L)以下;受试者尿样中芹菜素平均含量为(2251.72±1304.22)nmol/L 24 h,相当于摄入量的(0.62±0.37)%;红细胞中芹菜素含量在各时间点差异无统计学意义(P>0.05)。结论 通过食物摄入到体内的芹菜素一部分进入了人体循环系统,可以在体内发挥生物学作用。

Study on metabolism of apigenin from celery leaf in human

Objective Absorption and excretion of apigenin was tested after the ingestion of celery leaf.Methods Ten healthy subjects(5 women,5 men)in the age range of 24～28 years and with an average body mass index of 20.37 kg/m² took part in this study.After an apigenin-and luteolin-free diet,a single oral celery leaf of 2 g/(kg·bw)was consumed.Blood samples were taken at 4,6,7,8,9,10,11 and 28 h after celery leaf consumption and 24h urine samples were collected.Apigenin was analyzed in plasma,urine and red blood cells by means of HPLC-ECD.Results A maximum apigenin plasma concentration of(207.11±97.55)nmol/L was reached after(7.70±0.82)h.For all participants,plasma apigenin concentration rose after celery leaf ingestion and fell within 28h under the detection limit(2.32 nmol/L).The average apigenin content in urine was(2251.72±1304.22)nmol/L 24h corresponding to(0.62±0.37)% of the ingested dose.The apigenin could be detected in red blood cells without time-response characteristics.Conclusion A small portion of apigenin provided by food reaches the human circulation and,therefore,may reveal biological effects.