灯台树种子萌发特性的研究

目的 研究灯台树种子萌发的最适宜条件和储藏时间，为大量人工繁殖灯台树苗木提供借鉴。方法 不同温度，不同光照时间，不同光质及不同储藏时间，测量灯台树种子的萌发率。结果 灯台树种子萌发的适宜温度为30℃～40℃，适宜的光照时间为8h，适宜的光质为黄光和日光，储藏时间为5个月以内最好。结论 灯台树种子是高温萌发型。

Germination characters of seed of Alstonia scholaris

Object To develop an immunoassay method of species-specific protein for Radix Glycyrrhizae (RG) identification. Methods The crude drug of RG as a model was studied in this paper,the antigen of RG protein (RGP) was screened by Western-blot analysis using anti-RG serum. After species-specific protein,RGP was purified,and rabbit antiserum against RGP was prepared,the rabbit IgG of anti-RGP was isolated and biotinylated,then the sandwich enzyme-linked immuosorbent assay (ELISA) was developed. Results The method was only specific to RG,which was harvested from various areas or species,and showed excellent sensitivity and reproducibility. Conclusion The result suggests that the immunoassay method using specific antigen of RGP as a detection objective be a new potential for the unequivocal identification and quality control of RG.