| 卡维地洛对大鼠心室肌细胞L型Ca电流阻滞作用的实验研究 |
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| 引用本文： | 曹艳杰,王海昌,张荣庆,杜俊杰,胡涛,范延红,马彦卓. 卡维地洛对大鼠心室肌细胞L型Ca电流阻滞作用的实验研究[J]. 西北国防医学杂志, 2009, 30(2): 94-96 |
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| 作者姓名： | 曹艳杰 王海昌 张荣庆 杜俊杰 胡涛 范延红 马彦卓 |
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| 作者单位： | 1. 第四军医大学西京医院,心脏内科,陕西,西安,710032
2. 第四军医大学西京医院,创伤矫形外科研究所,陕西,西安,710032 |
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| 摘 要： | 目的：观察卡维地洛对大鼠心室肌细胞ICa,L电流的抑制作用，探讨卡维地洛抗心律失常的离子通道机制。方法：采用胶原酶酶解法分离大鼠心室肌细胞；采用膜片钳全细胞技术记录大鼠心室肌细胞ICa,L电流，观察细胞外液中加入卡维地洛后，ICa,L电流的变化。结果：对照组及卡维地洛组ICa,L密度分别为（pA／pF）：11．25±1．57，3．89±0．59（P〈0．05）；卡维地洛组ICa,L的I—V曲线向上方移位。结论：卡维地洛能够对抗心律失常的机理之一与它能够阻断ICa,L有关。
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| 关 键 词： | 卡维地洛 心律失常 钙通道电流 膜片钳技术 |
Inhibitory effect of carvedilol on L-type calcium channel current of rat ventricular myocytes |
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| Affiliation: | CAO Yan - jie, WANG Hai - chang, ZHANG Rong - qing, et al. ( Department of Cardiovasology, Xijing Hospital, Fourth Military Medical University, PLA , Xi＇an 710032, China) |
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| Abstract: | Objective ：To observe the inhibitory action of carvedilol on L- type calcium channel current （ICa,L） of ventricular myocytes in rats, and to explore the ionic channel mechanism of antiarrhythmic effect of carvedilol.Methods： The ICa,L of ventricle cardiomyocytes isolated by collagenase method was recorded by using patch clamp technique. The influence of carvedilol on ICa,L was analyzed. Results： The ICa,L current densities （pA/pF） were 11.25 ±1.57 in control group and 3.89 ± 0.59 in carvedilol group respectively （ P 〈 0.05 ）, and the current - voltage relationship （I- V） curve was shifted upward in carvedilol group. Conclusion：The antiarrhythmic effects of carvedilol may be related with the inhibition of ICa,L in ventricular myocytes. |
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| Keywords: | Carvedilol Arrhythmias Calcium channel current Patch clamp technique |
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