| 低氧预处理对大鼠BMSCs葡萄糖代谢的影响 |
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| 作者姓名： | Zhu H Chen X Deng L |
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| 作者单位： | 四川大学华西医院生物治疗国家重点实验室;四川大学华西医院再生医学研究中心·干细胞与组织工程研究室; |
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| 基金项目： | 国家高技术研究发展计划(863)资助项目(2007AA021900); 四川省科技支撑计划资助项目(2008SZ0035)~~ |
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| 摘 要： | 目的观察低氧预处理对BMSCs葡萄糖代谢的影响,探讨其潜在机制,为干细胞疗法的优化提供理论依据。方法取1～3日龄SD大鼠骨髓,采用密度梯度离心法获得BMSCs,取第4代细胞用于实验。根据处理方法不同将细胞分为4组:A组采用常氧培养24 h;B组以1%低氧浓度培养24 h;C组于低氧预处理前用20μmol/L甲氧雌二醇处理24 h;D组于低氧预处理前用50μmol/L缺氧诱导因子1(hypoxia-inducible factor 1,HIF-1)特异的siRNA处理12 h。采用MTT法、生化分析仪及实时荧光定量PCR检测各组BMSCs活力、葡萄糖代谢以及HIF-1αmRNA及葡萄糖转运蛋白1(glucose transporter 1,Glut-1)mRNA的表达。结果 MTT检测示A、B、C、D组吸光度(A)值分别为387.67±58.92、322.50±50.60、297.00±53.00、286.00±41.00,各组间差异无统计学意义(P>0.05)。与A组相比,B组葡萄糖摄取量和乳酸生成量显著增加(P<0.05);C、D组略高于A组,但差异无统计学意义(P>0.05),但显著低于B组,差异有统计学意义(P<0.05);C、D组间比较差异无统计学意义(P>0.05)。与A组相比,B组HIF-1αmRNA和Glut-1 mRNA表达均显著上调(P<0.05);C、D组HIF-1αmRNA和Glut-1 mRNA表达较B组大幅度降低(P<0.05),但仍显著高于A组(P<0.05);C、D组间差异无统计学意义(P>0.05)。结论低氧预处理可上调大鼠BMSCs的葡萄糖摄取和代谢能力,其机制涉及HIF-1 mRNA及其下游基因Glut-1 mRNA的表达上调。
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| 关 键 词： | BMSCs 低氧预处理 缺氧诱导因子1 葡萄糖转运蛋白1 大鼠 |
Effects of hypoxic preconditioning on glucose metabolism of rat bone marrow mesenchymal stem cells |
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| Zhu H,Chen X,Deng L.Effects of hypoxic preconditioning on glucose metabolism of rat bone marrow mesenchymal stem cells[J].Chinese Journal of Reparative and Reconstructive Surgery,2011,25(8):1004-1007. |
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| Authors: | Zhu Hongming Chen Xiaohe Deng Li |
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| Institution: | ZHU Hongming1,CHEN Xiaohe2,DENG Li2.1State Key Laboratory of Biotherapy,2Division of Stem Cell and Tissue Engineering,Regenerative Medicine Research Center,West China Hospital,Sichuan University,Chengdu Sichuan,610041,P.R.China |
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| Abstract: | Objective To study the effects of hypoxic preconditioning on the glucose metabolism of rat BMSCs and its underlying mechanism so as to provide the theoretical basis for the optimization of the stem-cell based therapy.Methods Density gradient centrifugation method was adopted to isolate rat BMSCs from neonatal SD rats(aged 1-3 days).BMSCs were cultured to 4th passage and divided into 4 groups based on di erent culture conditions: group A in normoxia condition for 24 hours,group B in 1% O2 for 24 hours,group ... |
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| Keywords: | Bone marrow mesenchymal stem cells Hypoxic preconditioning Hypoxia-inducible factor 1 Glucose transporter 1 Rat |
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