| 人Ⅰ型前胶原基因反义寡核苷酸对人增生性瘢痕裸鼠移植模型胶原合成的作用 |
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| 引用本文： | 袁即山,利天增,祁少海. 人Ⅰ型前胶原基因反义寡核苷酸对人增生性瘢痕裸鼠移植模型胶原合成的作用[J]. 中国修复重建外科杂志, 2011, 0(6) |
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| 作者姓名： | 袁即山 利天增 祁少海 |
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| 作者单位： | 江苏大学附属人民医院骨科;中山大学附属第一医院烧伤科; |
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| 基金项目： | 广东省重点科研项目(199827817); 广东省医学科研联合攻关项目(98002)~~ |
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| 摘 要： | 目的人Ⅰ型前胶原基因(ColⅠA1)反义寡核苷酸(antisense oligodeoxyneucleotide,ASODN)对体外培养的人增生性瘢痕成纤维细胞有抑制胶原合成作用,拟进一步探讨ColⅠA1 ASODN对裸鼠移植模型体内的人增生性瘢痕的胶原合成作用。方法 SPF级BALB/c-nunu品系6～8周龄雌性裸鼠60只,体重约20 g;取行瘢痕切除手术患者自愿捐赠的瘢痕组织块去表皮后,移植至裸鼠背部肩胛内侧皮下,每只裸鼠移植1块,制备人增生性瘢痕裸鼠移植模型。将58只成功制备模型的裸鼠根据处理方法不同,随机分成3组,于瘢痕移植2周根据分组行经皮穿刺瘢痕内注射。A组(n=20):5μL浓度为3 mmol/L ColⅠA1 ASODN、3μL脂质体、92μL Opti-MEMⅠ减血清培养基;B组(n=20):3μL脂质体、97μL Opti-MEMⅠ减血清培养基;C组(n=18):100μL Opti-MEMⅠ减血清培养基。实验最初2周每天注射1次,之后隔天1次,至实验取材。注射后2、4、6周,对存活裸鼠进行瘢痕硬度测量后,处死裸鼠取瘢痕组织行胶原染色组织学观察以及透射电镜观察;提取细胞总RNA后行RT-...
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| 关 键 词： | Ⅰ型前胶原基因 反义寡核苷酸 增生性瘢痕 胶原 裸鼠 |
EFFECT OF Col I A1 ANTISENSE OLIGODEOXYNEUCLEOTIDE ON COLLAGEN SYNTHESIS IN HUMAN HYPERTROPHIC SCAR TRANSPLANTED NUDE MOUSE MODEL |
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| YUAN Jishan,LI Tianzeng,QI Shaohai. EFFECT OF Col I A1 ANTISENSE OLIGODEOXYNEUCLEOTIDE ON COLLAGEN SYNTHESIS IN HUMAN HYPERTROPHIC SCAR TRANSPLANTED NUDE MOUSE MODEL[J]. Chinese journal of reparative and reconstructive surgery, 2011, 0(6) |
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| Authors: | YUAN Jishan LI Tianzeng QI Shaohai |
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| Affiliation: | YUAN Jishan1,LI Tianzeng2,QI Shaohai2.1Department of Orthopedics,the Affiliated People's Hospital of Jiangsu University,Zhenjiang Jiangsu,212002,P.R.China,2Department of Burns,the First Affiliated Hospital,Sun Yat-sen University. |
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| Abstract: | Objective Col I A1 antisense oligodeoxyneucleotide(ASODN) has inhibitory effect on collagen synthesis in cultured human hypertrophic scar fibroblasts.To investigate the effects of intralesional injection of Col I A1 ASODN on collagen synthesis in human hypertrophic scar transplanted nude mouse model.Methods The animal model of human hypertrophic scar transplantation was established in the 60 BALB/c-nunu nude mice(specific pathogen free grade,weighing about 20 g,and aged 6-8 weeks) by transplanting hypertrop... |
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