胜利油田地区德国小蠊抗药性监测结果分析

目的了解山东省胜利油田地区德国小蠊抗药性现状,为有效杀灭德国小蠊提供科学策略。方法采用药膜法监测胜利油田不同地区德国小蠊对常用化学杀虫剂的抗药性;应用酶试剂盒测定谷胱甘肽S-转移酶(GSTs)、乙酰胆碱酯酶(AChE)和超氧化物歧化酶(SOD)的活性。结果胜利油田地区东营、河口、孤岛、仙河4个地区德国小蠊对溴氰菊酯的抗性倍数最高,分别为25.76、16.68、15.77、14.21;其次是氯氰菊酯,抗性倍数分别为23.72、8.34、8.05、7.81;不同地区野外品系德国小蠊分别与敏感品系酶活力比较,东营、孤岛、仙河野外品系德国小蠊的GSTs酶活力分别为(75.97±4.16)、(58.87±2.88)、(59.51±2.73)U/mgprot,均高于敏感品系的(53.16±2.25)U/mgprot,东营、河口、孤岛、仙河野外品系德国小蠊的AChE酶活力分别为(1.22±0.07)、(1.01±0.06)、(0.97±0.11)、(0.77±0.04)U/mgprot,均高于敏感品系的(0.39±0.01)U/mgprot,东营野外品系德国小蠊的SOD酶活力为(33.24±1.53),高于敏感品系的(29.18±1.25)U/mgprot,差异均有统计学意义(均P<0.05)。结论胜利油田地区野外品系德国小蠊对溴氰菊酯和氯氰菊酯的抗药性较高, GSTs和AChE相对活性可作为德国小蠊抗药性的监测指标。

Insecticide resistance in Blattella germanica found in areas of Shengli oil field

ObjectiveTo study the status of insecticide resistance in Blattella germanica found in Shengli oil field and to provide evidences for effective control of the insects.MethodsThe resistance to common chemical insecticides in Blattella germanica found in different areas of Shengli oil field was determined with residual film method and the activities of glutathione S-transferase(GSTs),acetylcholin esterase(AChE),and superoxide dismutase(SOD) in the Blattella germanica were also detected with enzyme reagent kits.ResultsFor the Blattella germanica(field strain) trapped in 4 areas of Shengli oil field,the resistance to deltamethrin was the highest,with the resistance ratios of 14.21-25.76,followed by that to cypermethrin,acephate,and propoxur,with the resistance ratios of 7.81-23.72,5.97-7.08,and less than 2.The relative activities of GSTs(107%-143%) and AChE(197%-312%) in the Blattella germanica were all higher than those of the sensitive strain,but the relative activities of SOD(102%-114%) showed no obvious difference compared to that of the sensitive strain.ConclusionThe insecticide resistance in Blattella germanica(field strain) found in areas of Shengli oil field is high and relative activity of GSTs and AChE could be used in as indicators of insecticide resistance in Blattella germanica.