首页 | 本学科首页   官方微博 | 高级检索  
     

氟对SH-SY5Y细胞活性氧水平和线粒体融合影响
引用本文:楼迪栋, 郑丹, 潘际刚, 张凯琳, 罗亚, 于燕妮, 官志忠. 氟对SH-SY5Y细胞活性氧水平和线粒体融合影响[J]. 中国公共卫生, 2014, 30(6): 755-757. DOI: 10.11847/zgggws2014-30-06-19
作者姓名:楼迪栋  郑丹  潘际刚  张凯琳  罗亚  于燕妮  官志忠
作者单位:1.贵阳医学院病理学教研室, 贵州 贵阳 550000;2.贵阳医学院法医学系;3.贵阳市妇幼保健院;4.贵州省分子生物学重点实验室
基金项目:国家科技支撑计划(2013BAI05B03);国家自然科学基金(81160335);贵州省科学技术基金(黔科合J字(2013)2036号);温州市科学技术基金(Y20080099)
摘    要:目的观察氟对人脑神经母细胞瘤细胞株(SH-SY5Y细胞)体外培养细胞活性氧水平和线粒体融合的影响。方法在SH-SY5Y细胞中加入0.4、4 mmol/L NaF,孵育6、12、24和48 h;利用MitoTracker和ROS荧光染色试剂盒对线粒体和氧自由基进行染色。结果NaF作用6、12、24、48 h时,与对照组比较,0.4、4 mmol/L氟处理组SH-SY5Y细胞内ROS染色强阳性细胞计数[分别为(15.1±1.4)、(12.8±2.5)、(22.6±2.4)、(30±3.1)和(32.5±1.9)、(38.1±2.2)、(69.3±3.1)、(84.3±3.5)个/100细胞]和Ⅱ型线粒体细胞计数[分别为(13±1.4)、(15±1.7)、(22.9±1.9)、(23.7±1.6)和(15.2±2.3)、(23.8±1.8)、(29.1±4)、(47.7±3.3)个/100细胞]明显升高(P<0.05)。结论摄入过量的氟导致SH-SY5Y细胞氧自由基水平升高,线粒体呈分裂状,呈时间和剂量效应关系。

关 键 词:氟化物中毒  活性氧  线粒体  SH-SY5Y细胞
收稿时间:2013-05-23

Changes of ROS level and mitochondria fission-fusion in SH-SY5Y cells with chronic fluorosis
LOU Di-dong, ZHENG Dan, PAN Ji-gang.et al, . Changes of ROS level and mitochondria fission-fusion in SH-SY5Y cells with chronic fluorosis[J]. Chinese Journal of Public Health, 2014, 30(6): 755-757. DOI: 10.11847/zgggws2014-30-06-19
Authors:LOU Di-dong  ZHENG Dan  PAN Ji-gang.et al
Affiliation:1.Department of Pathology, Guiyang Medical College, Guiyang, Guizhou Province 550004, China
Abstract:ObjectiveTo investigate the changes of reactive oxygen species(ROS)level and mitochondrial fission-fusion in SH-SY5Y neurons with chronic fluorosis.MethodsNeuroblastoma SH-SY5Y cells were exposed to different concentrations of fluoride(0.04 and 4 mmol/L)in vitro to build cellular model of fluorosis with the exposure time of 6,12,24,and 48 hours,respectively.Mitochondrial morphology and ROS levels of SH-SY5Y stained by mito-tracker RED probe and ROS fluorescence dyes were observed with fluorescence microscope.ResultsCompared with untreatment cells,the numbers of ROS stain positive SY-SY5Y cells(0.4 mmol/L fluoride:15.1±1.4,12.8±2.5,22.6±2.4,30.0±3.1;4 mmol/L fluoride:32.5±1.9,38.1±2.2,69.3±3.1,84.3±3.5)and the numbers of abnormal mitochondria in morphology(0.4 mmol/L fluoride:13±1.4,15±1.7,22.9±1.9,23.7±1.6;4 mmol/L fluoride:15.2±2.3,23.8±1.8,29.1±4,47.7±3.3)were significantly increased after exposure of 6,12,24,and 48 hours(P<0.05).ConclusionTaking excessive amount of fluoride results in high level of oxidative stress and mitochondrial fragmentation,which are dependent on the exposure time and dose of fluoride.
Keywords:fluoride poisoning  reactive oxygen species  mitochondria  SH-SY5Y
点击此处可从《中国公共卫生》浏览原始摘要信息
点击此处可从《中国公共卫生》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号