海参粉对小鼠机体免疫功能影响

目的 探讨海参粉对正常小鼠免疫功能的调节作用。方法 无特定病原体(SPF)昆明种小鼠200只,雄性,随机分为4组,每组50只,即对照组、海参粉低、中、高剂量组(0.33、0.67、1.00 g/kg),连续灌胃30 d,采用噻唑蓝(MTT)法测定小鼠脾淋巴细胞增殖反应能力和自然杀伤(NK)细胞活性,观察二硝基氟苯(DNFB)诱导迟发型变态反应(DTH)和T淋巴细胞亚群影响;通过血清溶血素和抗体生成细胞(PFC)检测体液免疫功能;利用单核巨噬细胞和网状内皮系统的吞噬功能检测小鼠非特异性免疫功能。结果 与对照组比较,海参粉各剂量组小鼠体重增重、胸腺指数、脾脏指数、耳肿胀指数无明显变化;与对照组比较,低、中剂量海参粉组小鼠脾淋巴细胞转化能力[分别为(0.378±0.125)、(0.370±0.096)]与NK细胞活性[分别为(57.24±7.31)、(57.54±7.29)]均升高(P<0.05),低、高剂量海参粉组小鼠碳廓清试验吞噬指数α[分别为(6.18±0.86)、(6.25±1.21)]明显升高(P<0.05)。结论 海参粉对正常小鼠免疫功能有一定调节作用,可增强小鼠细胞免疫能力。

Effect of sea cucumber powder on immune function in mice

Objective To investigate regulative effects of sea cucumber powder on immune function in normal mice.Methods Totally 200 male specific pathogen free Kunming mice were divided randomly into four groups:a control group and high,moderate,and low treatment groups with 0.33,0.67,and 1.00 g/kg sea cucumber powder administered by oral gavage for 30 days.The splenic lymphocyte tranformation efficiency and activity of natural killer(NK)cells were determined with methylthiazolyldiphenyl-tetrazolium bromide(MTT)method.The delayed type hypersensitivity (DTH)and the impacts of T cell subgroups were induced with dinitrofluorobenzene(DNFB)method.The humoral immunity function was determined with serum antihemolysin and antibody-forming cell assay.Non-specific immune function was detected with monocaryon-macrophage cell and reticuloendothelial system phagocytosis.Results Compared with the control group,there were no significant changes in body weight in the mice of the three groups with sea cucumber powder treatment.There were no significant changes in thymus gland index,spleen index,and ear-swelling test.Compared with the control group,the splenic lymphocyte tranformation efficiency and NK cell activity were 0.378±0.125,0.370±0.096 and 57.24±7.31,57.54±7.29 for moderate and low dose treatment groups,and all were higher than those of the control group(P<0.05); the carbon clearance index of the mice in low and high dose treatment groups were 6.18±0.86 and 6.25±1.21,with significantl improvement(P<0.05).Conclusion Sea cucumber powder has some regulative effect on immune function and can enhance cellular immunity function in mice.