| 新型纳米纤维支架的细胞生物相容性 |
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| 引用本文： | 曹西迎,翁国星,肖荣冬,刘海清,王江南. 新型纳米纤维支架的细胞生物相容性[J]. 中国组织工程研究与临床康复, 2011, 15(21). DOI: 10.3969/j.issn.1673-8225.2011.21.008 |
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| 作者姓名： | 曹西迎 翁国星 肖荣冬 刘海清 王江南 |
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| 作者单位： | 1. 福建医科大学省立临床医学院心血管外科,福建省福州市,350001
2. 福建师范大学化学与材料学院、福建省高分子材料重点实验室,福建省福州市,350007 |
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| 摘 要： | 背景:高孔隙率聚己内酯纳米纤维支架具有适合血管平滑肌细胞黏附、增殖的多级孔径结构,具有良好的细胞生物相容性.目的:探讨高孔隙率聚己内酯静电纺丝纳米纤维支架的细胞相容性.方法:根据支架的制作工艺不同分为传统支架组、新型纳米纤维支架组两组,另设单纯细胞组为对照组.采用组织块贴壁法体外原代培养兔主动脉平滑肌细胞并进行传代,用3～6代细胞作为实验用种子细胞.应用WST-1法测定平滑肌细胞黏附率、增殖力,光镜及扫描电镜观察细胞形态,评估支架的细胞生物相容性.结果与结论:高孔隙率聚己内酯纳米纤维支架对细胞形态无明显影响,新型支架上的种子细胞黏附、增殖及代谢活性情况较传统支架好.提示,高孔隙率聚己内酯静电纺丝纳米纤维支架具有较高的细胞相容性.
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| 关 键 词： | 纳米纤维 生物材料 支架 组织工程 细胞相容性 |
Cell biocompatibility of a new-type nanofiber scaffold for tissue engineering |
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| Cao Xi-ying,Weng Guo-xing,Xiao Rong-dong,Liu Hai-qing,Wang Jiang-nan. Cell biocompatibility of a new-type nanofiber scaffold for tissue engineering[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(21). DOI: 10.3969/j.issn.1673-8225.2011.21.008 |
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| Authors: | Cao Xi-ying Weng Guo-xing Xiao Rong-dong Liu Hai-qing Wang Jiang-nan |
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| Affiliation: | Cao Xi-ying1,Weng Guo-xing1,Xiao Rong-dong1,Liu Hai-qing2,Wang Jiang-nan21Department of Cardiovascular Surgery,Fujian Provincial Hospital,Clinical Medical College,Fujian Medical University,Fuzhou 350001,Fujian Province,China,2College of Chemistry and Materials Science,Fujian Normal University,Key Laboratory of Polymer Materials of Fujian Province,Fuzhou 350007 |
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| Abstract: | BACKGROUND:High-porosity polycaprolactone(PCL) nanofiber scaffolds have a good multi-pore structure for the adhesion and proliferation of vascular smooth muscle cells and perfect biocompatibility.OBJECTIVE:To evaluate the cell biocompatibility of high-porosity PCL scaffold made by electrostatic spinning technology.METHODS:Electrostatic spinning technology was used to produce high-porosity PCL nanofiber stents.WST-1 method was used to evaluate the effects of scaffold on the adhesion and proliferation of smoo... |
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