| 高糖状态下小鼠肾小球足细胞凋亡和组成型光形态建成1蛋白的表达 |
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| 引用本文： | 王晓林,甘华,杜晓刚. 高糖状态下小鼠肾小球足细胞凋亡和组成型光形态建成1蛋白的表达[J]. 中国组织工程研究与临床康复, 2011, 15(24): 4461-4464. DOI: 10.3969/j.issn.1673-8225.2011.24.022 |
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| 作者姓名： | 王晓林 甘华 杜晓刚 |
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| 作者单位： | 重庆医科大学附属第一医院肾脏内科,重庆市,400016 |
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| 基金项目： | 课题受高等学校博士学科点专项科研基金(20070631008) 资助;S 抑制性COP1 介导的p53 泛素化降解防治糖尿病肾病大鼠肾小球足细胞凋亡 |
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| 摘 要： | 背景:组成型光形态建成1蛋白与细胞凋亡有关。目的:观察高糖对体外培养的小鼠肾小球足细胞凋亡和组成型光形态建成1蛋白表达的影响。方法:将不同浓度葡萄糖溶液分别加入体外培养的条件永生性小鼠肾小球足细胞株培养液中,培养不同时间后检测肾小球足细胞凋亡指数和死亡指数,以筛选最佳剂量效应葡萄糖浓度。用30mmol/L葡萄糖溶液干预小鼠肾小球足细胞(高糖组),并设立对照组和采用30mmol/L甘露醇干预的甘露醇组。结果与结论:在一定浓度范围内,葡萄糖呈时间和剂量依赖性诱导肾小球足细胞凋亡和死亡(P<0.05),随着葡萄糖浓度的进一步加大,肾小球足细胞死亡指数明显升高,而凋亡指数变化不大。与对照组比较,高糖组凋亡指数显著增加(P<0.05),其COP1 mRNA及蛋白表达水平均明显下降(P<0.05)。结果证实,高糖可诱导肾小球足细胞的凋亡,其机制可能与其下调COP1的表达有关。
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| 关 键 词： | 肾小球足细胞 高糖 组成型光形态建成1蛋白 凋亡 组织构建 |
High glucose induces podocyte apoptosis and constitutive photomorphogenic 1 expression changes |
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| Wang Xiao-lin,Gan Hua,Du Xiao-gang. High glucose induces podocyte apoptosis and constitutive photomorphogenic 1 expression changes[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(24): 4461-4464. DOI: 10.3969/j.issn.1673-8225.2011.24.022 |
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| Authors: | Wang Xiao-lin Gan Hua Du Xiao-gang |
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| Affiliation: | Wang Xiao-lin,Gan Hua,Du Xiao-gang Department of Nephrology,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China |
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| Abstract: | BACKGROUND:Studies have shown that constitutive photomorphogenic 1(COP1) is involved in cell apoptosis.OBJECTIVE:To observe the effects of high glucose exposure on expression of in vitro cultured podocyte apoptosis and COP1 expression.METHODS:Mouse podocytes of an immortalized cell line were treated with different concentrations of glucose.Apoptosis index and mortality index at different culture time were evaluated by flow cytometry to screen best dose-effect glucose concentration(30 mmol/L),which was used ... |
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