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| 人脐带间充质干细胞向成骨细胞及脂肪细胞分化过程中nm23-H1基因的表达 | |
| 引用本文： | 荣靖,彭鑫磊,马岩岩,刘秋英,周向荣,罗新,王一飞.人脐带间充质干细胞向成骨细胞及脂肪细胞分化过程中nm23-H1基因的表达[J].中国组织工程研究与临床康复,2011,15(19):3467-3471. |
| 作者姓名： | 荣靖 彭鑫磊 马岩岩 刘秋英 周向荣 罗新 王一飞 |
| 作者单位： | 1. 广州(暨南)生物医药研究开发基地,广东省广州市,510632 2. 亚太干细胞科研中心有限公司,香港 3. 暨南大学附属第一医院妇产科,广东省广州市,510632 |
| 基金项目： | 国际科技合作项目，广州(暨南)-香港细胞工程联合实验室项目，国家自然科学基金，中央高校基本科研业务费专项资金资助 |
| 摘 要： | 背景:nm23-h1基因已被证实与多种细胞的增殖、分化、转移密切相关。目的:观察人脐带间充质干细胞在体外向成脂及成骨方向诱导分化的过程中nm23-H1基因的表达变化。方法:采用组织块贴壁法从脐带中分离培养人间充质干细胞,流式细胞术鉴定其表型,之后分别加入成脂肪诱导剂和成骨诱导剂进行体外诱导分化。对照组采用不含诱导因子的培养液进行培养。结果与结论:在成脂诱导过程的第4天,nm23-H1 mRNA的表达上调,至第7天达到最高(P<0.01),其后开始逐渐恢复,直至第14天与对照组相比无显著差异(P>0.05)。而在成骨诱导过程中,nm23-H1 mRNA则一直为低表达状态(P<0.01),直至第28天恢复为对照组水平(P>0.05)。结果显示nm23-H1基因表达在成脂分化前期发生上调,而在成骨分化过程中一直下调。 |
| 关 键 词： | 脐带间充质干细胞 脂肪细胞 成骨细胞 分化 nm 23-H1 |
Expression of nm23-H1 during differentiation of human umbilical cord mesenchymal stem cells into adipocytes and osteoblasts in vitro |
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| Rong Jing,Peng Xin-lei,Ma Yan-yan,Liu Qiu-ying,Zhou Xiang-rong,Luo Xin,Wang Yi-fei.Expression of nm23-H1 during differentiation of human umbilical cord mesenchymal stem cells into adipocytes and osteoblasts in vitro[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(19):3467-3471. | |
| Authors: | Rong Jing Peng Xin-lei Ma Yan-yan Liu Qiu-ying Zhou Xiang-rong Luo Xin Wang Yi-fei |
| Institution: | Rong Jing1,Peng Xin-lei1,Ma Yan-yan1,Liu Qiu-ying1,Zhou Xiang-rong 2,Luo Xin3,Wang Yi-fei1 1Guangzhou (Jinan) Biomedicine Research & Development Center,Guangzhou 510632,Guangdong Province,China,2Asia Pacific Stem Cell Science Limited,Hong Kong,3Department of Obstetrics and Gynecology,First Affiliated Hospital of Jinan University |
| Abstract: | BACKGROUND: It has been proved that nm23-H1 gene has a great infulence on proliferation,differentiation and transfering of many kinds of cells.OBJECTIVE: To study on the expression of nm23-H1 gene during differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) into adipocytes and osteoblasts in vitro.METHODS: The hUCMSCs were isolated from human umbilical cord by tissue adherence,and a flow cytometry was used for immunophenotype identification.Then the 3rd passage cells were induced to diffe... |
| Keywords: | nm23-H1 |
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