| 天然与重组水蛭素对大鼠随意皮瓣淤血模型超氧化物歧化酶、丙二醛、内皮素变化的影响 |
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| 引用本文： | 郭应信,殷国前,李佳荃.天然与重组水蛭素对大鼠随意皮瓣淤血模型超氧化物歧化酶、丙二醛、内皮素变化的影响[J].中国组织工程研究与临床康复,2011,15(24):4453-4456. |
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| 作者姓名： | 郭应信 殷国前 李佳荃 |
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| 作者单位： | 广西医科大学第一附属医院整形美容外科,广西壮族自治区南宁市,530021 |
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| 基金项目： | 2010 年广西自然科学基金面上项目，天然、重组水蛭素对随意皮瓣淤血模型大鼠中VEGF 、CD34 的影响 |
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| 摘 要： | 背景:一般认为天然水蛭素能够提高淤血皮瓣的成活率与其抗血栓、抗凝血酶有关,但其抗炎、抗氧化作用的具体机制尚不清晰。重组水蛭素效果与天然水蛭素的差异也未有深入研究。目的:探索天然、重组水蛭素对大鼠随意皮瓣淤血模型存活保护机制。方法:Wistar大鼠30只,背部设计随意皮瓣10cm×3cm制成淤血模型。皮瓣随机分为天然水蛭素组、重组水蛭素组和对照组,均于术后即刻、第3,5天注射在距皮瓣末端1.5cm和3.0cm处分别注射5U天然水蛭素、5U重组水蛭素和相同剂量生理盐水。观察术后第7天的皮瓣成活率、皮瓣组织细胞形态学改变以及超氧化物歧化酶、丙二醛、内皮素水平。结果与结论:与对照组相比,天然、重组水蛭素组皮瓣成活率高,皮瓣超氧化物歧化酶水平明显增多,内皮素、丙二醛水平减少。且天然水蛭素效果优于重组水蛭素。说明水蛭素能够提高随意皮瓣的成活率、超氧化物歧化酶的含量;减低皮瓣的坏死率、丙二醛、内皮素的含量。
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| 关 键 词： | 天然水蛭素 重组水蛭素 超氧化物歧化酶 丙二醛 内皮素 |
Effect of natural and recombinant hirudin on superoxide dismutase, malonaldehyde and endothelin in rat random skin flap congestion models |
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| Guo Ying-xin,Yin Guo-qian,Li Jia-quan.Effect of natural and recombinant hirudin on superoxide dismutase, malonaldehyde and endothelin in rat random skin flap congestion models[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(24):4453-4456. |
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| Authors: | Guo Ying-xin Yin Guo-qian Li Jia-quan |
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| Institution: | Guo Ying-xin,Yin Guo-qian,Li Jia-quan Department of Plastic and Aesthetic Surgery,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China |
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| Abstract: | BACKGROUND:Most scholars believed that the effect of hirudin can improve the survival rate of congestion flap based on its antithromotic and antithrombin activity.However,whether it has anti-inflammatory and antioxidant effects remains unclear.The disparity between recombinant hirudin and recombinant hirudin needs to be explored.OBJECTIVE:To investigate the protective effect of natural hirudin and recombinant hirudin on random skin flap survival in rats vein congestion models.METHODS:Thirty Wistar rats were... |
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