纳米银-猪小肠黏膜下层与聚丙烯网片修复兔腹壁损伤对比

背景:因为自体取材修复腹壁缺损是以伤治伤,而且取材有限,限制了临床应用,因此寻找理想的腹壁替代材料至关重要目的:比较复合修复材料纳米银-猪小肠黏膜下层与聚丙烯网片在腹壁缺损中的应用效果。方法:大耳兔制备兔腹壁损伤实验模型后随机数字表法分为2组,实验组应用复合材料修复腹壁缺损;对照组应用聚丙烯网片修复腹壁缺损。观察修复后动物一般恢复情况,于修复后1,2,4个月进行腹壁抗张强度测定、腹腔内粘连评分以及组织学检测。结果与结论:相同时间点实验组粘连度显著低于对照组(P<0.05);各组4个月腹腔粘连度均较1个月增加(P<0.05)。两组抗拉力强度修复后4个月均高于修复后1个月(P<0.01);实验组修复后1,2个月抗拉力强度高于对照组(P<0.05),到第4个月时,修补处强度几乎相等(P>0.05)。提示两种修复材料均具有良好的修复能力,修复后均能维持足够的腹壁抗张强度。但复合材料在组织相容性、抗腹腔粘连方面优于聚丙烯网片。自制的纳米银-猪小肠黏膜下层补料无细胞毒性,作为组织工程材料修复腹壁损伤具有可行性。

Nano silver-porcine small intestine submucosa and polypropylene mesh for repair of rabbit abdominal injury: A comparative study

BACKGROUND:It is essential to find the ideal replacement materials for abdominal injury.OBJECTIVE:To compare the application effect of composite repair materials-nano silver-porcine small intestine submucosa and polypropylene mesh on abdominal injury in rabbits.METHODS:Composite repair materials-nano silver-porcine small intestine submucosa,experimental models(3×3cm)with abdominal injury in rabbits caused by operation were prepared.Twenty white rabbits were randomly divided into experimental and control groups.Composite materials were used to repair abdominal injury in experimental group;polypropylene mesh was used to repair abdominal injury in control group.Postoperative recovery of rabbits was observed and the cytotoxicity of composite materials was evaluated after operation.Rabbits underwent tensile strength of abdominal wall determination,intra-abdominal adhesion score,and histological observation at 1,2 and 4 months after repair.RESULTS AND CONCLUSLON:Two groups were simultaneously compared after operation,the extent and intensity of abdominal adhesions in composite materials were significantly lower than that in polypropylene mesh at the same time point(P<0.05).The extent and intensity of abdominal adhesions in the two groups were higher at 4 months after repair than at 1 month after repair(P<0.05).The tensile strength of composite materials was higher than that of polypropylene mesh at 1 and 2 months after repair(P<0.05);and up to the fourth month,the tensile strength was similar in the two groups(P<0.05).These two repair materials have good repair capacity,which can maintain adequate tensile strength of abdominal wall after operation.However,composite materials in terms of histocompatibility and anti-abdominal adhesion are superior to polypropylene mesh.The self-made nano silver-porcine small intestine submucosa feeding has no cytotoxicity,and which has feasibility of repairing abdominal injury as tissue engineering material.