| 人参皂苷Rd干预体外培养肝储脂细胞胶原的表达 |
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| 引用本文： | 谯时文,王继丰,黄勤,朱宗贵,张知贵. 人参皂苷Rd干预体外培养肝储脂细胞胶原的表达[J]. 中国组织工程研究与临床康复, 2011, 15(24): 4457-4460. DOI: 10.3969/j.issn.1673-8225.2011.24.021 |
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| 作者姓名： | 谯时文 王继丰 黄勤 朱宗贵 张知贵 |
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| 作者单位： | 1. 乐山职业技术学院护理系解剖教研室,四川省乐山市,614000
2. 泸州医学院解剖学教研室,四川省泸州市,646000 |
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| 摘 要： | 背景:肝储脂细胞产生胶原是导致肝硬化的直接原因,调控肝储脂细胞产生胶原的能力即可以防治肝硬化。目的:观察人参皂苷Rd对体外培养肝储脂细胞Ⅰ,Ⅲ型胶原表达的影响。方法:体外培养肝储脂细胞,随机分为对照组和实验组,实验组用100mg/L人参皂苷Rd进行培养干扰,用免疫组织化学、免疫荧光技术检测Ⅰ,Ⅲ型胶原基因的表达。结果与结论:实验组比对照组,Ⅰ,Ⅲ型胶原阳性产物吸光度和面积比值明显降低(P<0.05),说明人参皂苷Rd对体外培养肝储脂细胞Ⅰ,Ⅲ型胶原的表达有明显调节作用,影响肝储脂细胞产生胶原纤维。
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| 关 键 词： | 人参皂苷 肝 储脂细胞 胶原 免疫组织化学 免疫荧光 |
Effect of ginsenoside Rd on collagen expression in liver fat-storing cells |
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| Qiao Shi-wen,Wang Ji-feng,Huang Qin,Zhu Zong-gui,Zhang Zhi-gui. Effect of ginsenoside Rd on collagen expression in liver fat-storing cells[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 15(24): 4457-4460. DOI: 10.3969/j.issn.1673-8225.2011.24.021 |
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| Authors: | Qiao Shi-wen Wang Ji-feng Huang Qin Zhu Zong-gui Zhang Zhi-gui |
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| Affiliation: | Qiao Shi-wen1,Wang Ji-feng2,Huang Qin1,Zhu Zong-gui1,Zhang Zhi-gui1 1Department of Anatomy,Leshan Vocational and Technical College Leshan 614000,Sichuan Province,China,2Department of Anatomy,Luzhou Medical College,Luzhou 646000 |
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| Abstract: | BACKGROUND:Collagens produced by liver fat-storing cells are the directly reasons for cirrhosis,thus,regulating capacity of fat-storing cells producing collagens can prevent cirrhosis.OBJECTIVE:To explore effect of ginsenoside Rd on expression of type Ⅰ and Ⅲ collagens in liver fat-storing cells in vitro.METHODS:The liver fat-storing cells were cultured in vitro and interfered with ginsenoside Rd.The expression of type Ⅰ and Ⅲ collagens was detected by immunohistochemistry and immunofluorescent.RESULTS AND CONCLUSION:Compared with the control group,the positive/negative-area and the absorbance of the experimental group were obviously decreased(P < 0.05),suggesting that ginsenoside Rd regulates the expression of type Ⅰ and Ⅲ collagens in liver fat-storing cells in vitro,which affects liver fat-storing cells producing collagens. |
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