补肾中药有效成分对大鼠骨损伤愈合及血液流变学的影响

背景:补肾中药为骨伤科常用药,但对不同有效成分用于骨愈合的比较研究尚缺乏.目的:观察不同补肾中药有效成分对骨损伤大鼠骨愈合及血液流变学的影响.方法:建立大鼠股骨干骨折模型并分别用提取的骨碎补总黄酮、淫羊藿总黄酮、菟丝子总黄酮、柚皮甙、槲皮素,以及橙皮甙对造模大鼠进行灌胃治疗.21 d 后取血及骨标本,检测大鼠骨愈合程度和血液流变学指标.结果与结论:不同补肾中药有效成分均对骨愈合有益,且骨碎补总黄酮、淫羊藿总黄酮效果优于其他4 种药物.骨碎补总黄酮和淫羊藿总黄酮可有效抑制低剪切速下大鼠的血液黏度,降低红细胞聚集指数及血小板聚集性和黏附性(P < 0.05),但对红细胞变形性无显著作用.说明补肾中药有效成分可促进骨损伤的愈合,且对血液流变学有一定作用,以骨碎补总黄酮效果最佳.

Influence of effective ingredients of Chinese kidney-tonifying drugs on rat bone healing and hemorheology

BACKGROUND: Chinese kidney-tonifying herb is commonly used in orthopedics department. However, individual effects of different ingredients remain poorly understood. OBJECTIVE: To observe influence of effective ingredients of Chinese kidney-tonifying drugs on bone healing and hemorheology of rats with bone injury. METHODS: Rat model of fracture of shaft of the femur was established, and treated by intragastric administration separately with extractive rhizoma drynariae total flavonoids, epimedium total flavonoids, dodder total flavonoids, naringin, quercetin and hesperidin. Blood and bone samples were harvested after 21 days to detect the extent of bone healing and hemorheology indexes. RESULTS AND CONCLUSION: The effective constituents of different Chinese kidney-tonifying drugs were beneficial to bone healing, and the effect of rhizoma drynariae total flavonoids and epimedium total flavonoids was better than the other drugs. Rhizoma drynariae total flavonoids and epimedium total flavonoids effectively inhibited blood viscosity under low shear, and reduced erythrocyte aggregation index and the aggregation and adhesion of platelet (P < 0.05), but it had no evident effects on erythrocyte deformation. This shows that the effective ingredient of the Chinese kidney-tonifying drugs can promote bone healing and exert certain effects on hemorheology. In particular, rhizoma drynariae total flavonoids exhibits the best effect.