Twist对胃癌细胞系MKN28迁移和侵袭能力的影响

目的:研究Twist基因对人胃癌细胞株MKN28迁移和侵袭能力的影响.方法:利用脂质体介导转染技术和遗传霉素筛选得到稳定高表达的Twist蛋白的细胞克隆Twist -MKN28;采用Western免疫印迹检测细胞中Twist,上皮细胞钙黏蛋白(ecadherin)和波形蛋白(vimentin)的表达;用基质胶侵袭小室检测细胞迁移和侵袭能力的影响.结果:阳性质粒组Twist -MKN28细胞的Twist蛋白表达强于空白质粒组PcDNA3.1-MKN28和未转染组MKN28;Twist -MKN28组细胞中ecadherin蛋白减少,而vimentin蛋白表达上调;Twist -MKN28组细胞迁移、侵袭能力明显增强.结论:Twist基因可能通过上皮-间质转型促进胃癌细胞的迁移和侵袭.

Effect of Twist gene on the migration and invasion of gastric carcinoma cells

OBJECTIVE: To explore the effect of Twist gene on the migration and invasion of human gastric carcinoma cells. METHODS: MKN28 cells, a human gastric carcinoma cell line, were transfected with PcDNA3.1-Twist plasmid by lipofectamine transfecting technique. The transfected cells were selected with geneticin. Expressions of Twist,ecadherin and vimentin protein were detected by Western blot in cells transfected Twist gene. Matrigel invision chambers were performed to analyse the cell migration and invasion. RESULTS: MKN28 cells transfected with PcDNA3.1-Twist plasmid showed stronger intracellular expression of Twist protein than MKN28 cells transfected with PcDNA3.1 and MKN28 cells without transfection. The expression of ecadherin protein in MKN28 cells transfected with PcDNA3.1-Twist plasmid was significantly decreased compared with that in MKN28 cells transfected with PcDNA3.1 and MKN28 cells without the transfection. However, The expression of vimentin protein in MKN28 cells transfected with PcDNA3.1-Twist plasmid was significantly increased compared with that in MKN28 cells transfected with PcDNA3.1 and MKN28 cells without transfection. The migration and invasion ability of Twist+ - MKN28 cells were stronger than that of MKN28 cells transfected with PcDNA3.1 and MKN28 cells without transfection. CONCLUSION: Twist gene may promote the migration and invasion ability of gastric carcinoma cells through epithelial mesenchymal transition.