| 丙泊酚后处理对大鼠肝缺血再灌注损伤时Bcl-2、Bax蛋白表达的影响 |
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| 引用本文： | 刘晓利,曹定睿,阎旭艳.丙泊酚后处理对大鼠肝缺血再灌注损伤时Bcl-2、Bax蛋白表达的影响[J].中国医疗前沿,2011(18):18-19. |
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| 作者姓名： | 刘晓利 曹定睿 阎旭艳 |
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| 作者单位： | 太原山西医科大学;山西医科大学第一临床医学院麻醉科; |
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| 摘 要： | 目的探讨丙泊酚后处理对大鼠肝缺血再灌注损伤的影响。方法成年Wistar大鼠60只,随机分为3组：假手术组（S组,n=12）、缺血再灌注组（IR组,n=12）、丙泊酚后处理组（P组,n=36）,丙泊酚后处理分为三个亚组（P1,P2,P4组）。制作70%肝脏缺血再灌注模型,实验结束后即刻取肝左叶做标本。用免疫组化法检测Bcl-2与Bax,TUNEL法检测肝细胞凋亡指数（AI）,光镜观察肝组织形态学变化。结果与S组比较,各组Bcl-2、Bax蛋白含量、AI均增加（P〈0.01）;与IR组比较,P1、P2、P4组Bcl-2蛋白表达增加、Bax蛋白表达和AI减少（P〈0.01）;与P4组比较,P1、P2组Bcl-2蛋白表达增加、Bax蛋白表达和AI减少（P〈0.01）。病理检查显示P1、P2、P4组肝组织结构损伤明显小于IR组。结论丙泊酚后处理可调节Bcl-2、Bax蛋白表达从而减轻肝细胞凋亡,临床剂量丙泊酚对大鼠缺血再灌注肝脏有一定的保护作用。
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| 关 键 词： | 丙泊酚 后处理 缺血再灌注损伤 肝脏 Bcl-2 Bax |
The effects of propofol postconditioning on expressions of Bcl-2 and Bax protein in hepatocytes after ischemia reperfusion injury in rats |
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| LIU Xiao-li,CAO Ding-rui,YAN Xue-yan.The effects of propofol postconditioning on expressions of Bcl-2 and Bax protein in hepatocytes after ischemia reperfusion injury in rats[J].China Healthcare Innovation,2011(18):18-19. |
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| Authors: | LIU Xiao-li CAO Ding-rui YAN Xue-yan |
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| Institution: | LIU Xiao-li,CAO Ding-rui,YAN Xue-yan.Department of Anesthesiology,First Clinical Medical College,Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective To investigate the effects of propofol postconditioning on the ischemia reperfusion injury(IR) in rat livers.Methods Sixty Wistar rats were randomly divided into 3 groups: sham operation group(S group,n=12),ischemia-reperfusion group(IR group,n=12),propofol postconditioning group(P group,n=36).Propofol postconditioning group were further divided into 3 groups(P1,P2,P4).A rat model of hepatic ischemia-reperfusion was established.The left hepatic lobes were sampled at the end of reperfusion.Bcl-2 an... |
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| Keywords: | Propofol Postconditioning Ischemia-reperfusion injury Liver Bcl-2 Bax |
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