湖北地区宫颈癌组织HPV16 E7和E5转化基因变异分析

目的:分析湖北地区宫颈癌组织HPV16E7和E5基因序列变异情况。方法:对87例宫颈鳞癌组织分别采用多重HPV16E7引物进行巢式PCR扩增和HPV16E5特异引物进行PCR扩增,并选择阳性扩增的基因片段DNA进行纯化、测序,检测基因变异。结果:测序成功的20例宫颈癌组织DNA中,HPV16E7基因的5个突变位点为:T846C、A647G、T732C、T789C、T795G,突变频率分别为85%(17/20)、70%(14/20)、15%(3/20),10%(2/20)、5%(1/20);HPV16E5基因的4个突变位点包括:A3979C、A4042G、G3868A、C3991T,突变频率分别为:80%(16/20)、90%(18/20)、5%(1/20)、5%(1/20)。结论:湖北地区宫颈癌的宫颈组织中HPV16E7热点突变为A647G和T846C,HPV16E5的热点突变为A3979C和A4042G,本研究为湖北地区宫颈癌流行病学的研究打下基础。

Polymorphisms of E7 and E5 Genes in Human Papillomavirus Type 16 Among Patients with Cervical Cancer in Hubei Province of China

Objective:To identify the sequence variations in the HPV 16 E7 and E5 genes among patients with cervical cancer in Hubei province of China. Methods:Eighty-seven patients with cervical cancer were involved in this study. Using polymerase chain reaction (PCR) and PCR-directed sequencing methods,the variations of the HPV 16 E7 and E5 genes were investigated. Results:The rank orders of incidence of HPV16 E7 variants from cervical cancer in Hubei were as follows:T846C in 85% (17/20),A647G in 70% (14/20),T732C in 15% (3/20),T789C in 10% (2/20),and T795G in 5% (1/20). There are four points of mutation in E5 gene,including A3979C,A4042G,G3868A,and C3991T with mutant frequencies of 80%(16/20),90%(18/20),5%(1/20),and 5%(1/20),respectively. Conclusion:The hot-spot mutations of HPV16 from cervical cancer in Hubei are A647G and T846C in E7 gene,and A3979C and A4042G in HPV16 E5 gene. The characterization of sequence variations within high-risk HPV 16 might be important in the search for epidemiological cervical cancer risk factors.