| 先天性肛门直肠畸形胎鼠肠管BMP4表达及意义 |
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| 引用本文： | 陈新新,陈兰萍,任红霞,吴晓霞,陈淑芸.先天性肛门直肠畸形胎鼠肠管BMP4表达及意义[J].中国医疗前沿,2011(19):39-41. |
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| 作者姓名： | 陈新新 陈兰萍 任红霞 吴晓霞 陈淑芸 |
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| 作者单位： | 山西医科大学儿科系;山西省儿童医院; |
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| 基金项目： | 山西省卫生厅科技攻关计划项目(编号:200937) |
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| 摘 要： | 目的研究先天性肛门直肠畸形（ARMs）胎鼠小肠、结肠和直肠骨形成蛋白4（BMP4）的表达情况,并探讨其在自身肠神经系统（ENS）发育过程中的作用。方法利用全反式维甲酸（ARTA）诱导大鼠产生肛门直肠畸形胚胎,孕20d剖宫取胎,选取对照组正常胎鼠12只,实验组ARMs胎鼠24只,在大体显微镜下解剖整个肠管,选取距盲肠10cm处小肠,距盲肠3cm处结肠,距肛门0.5cm处直肠各一段,应用SP免疫组化方法检测对照组和实验组小肠、结肠和直肠BMP4的表达情况。结果对照组肠壁肌间及粘膜下神经丛可见BMP4抗体染色阳性细胞,实验组胎鼠小肠阳性细胞的MOD值与对照组相比,差异无统计学意义（P〉0.05）;而实验组胎鼠直肠、结肠的阳性细胞的MOD值与对照组相比明显减小,差异有统计学意义（P〈0.05）。结论ARMs胎鼠结肠、直肠BMP4表达水平减低,可能与其远端肠管ENS发育不良有关。
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| 关 键 词： | 肛门直肠畸形 肠神经系统 骨形成蛋白4 |
Expression of BMP4 in guts of fetal rats with anorectal malformations |
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| CHEN Xin-xin,CHEN Lan-ping,REN Hong-xia,et al..Expression of BMP4 in guts of fetal rats with anorectal malformations[J].China Healthcare Innovation,2011(19):39-41. |
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| Authors: | CHEN Xin-xin CHEN Lan-ping REN Hong-xia |
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| Institution: | CHEN Xin-xin,CHEN Lan-ping,REN Hong-xia,et al.Department of Paediatric Surgery,Shanxi Medical University,Taiyuan 030001,China Corresponding author: CHEN Lan-ping |
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| Abstract: | Objective Explore the expression of bmp4 in small intestine,colon and rectum in ARTA-induced fetal rats with ARMs on embryonic day 20,to investigate the role of BMP4 during the ENS(enteric nervous system) development.Methods The fetal rats with ARMs were induced by ARTA,embryos were harvested via caesarean section on E20,whole intestine including small intestine,colon and rectum were eviscerated(there were 12 specimens in control group and 24 specimens in ARMs group).Sections were stained with bmp4 antibody... |
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| Keywords: | Anorectal malformations Enteric nervous system Bone morphogenetic proteins-4 |
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