超声波细胞粉碎仪与佐剂性关节炎模型的建立

目的：比较利用超声波细胞粉碎仪乳化弗氏完全佐剂（FCA）与单纯FCA诱导大鼠产生佐剂性关节炎（adiuvant arthritis，AA）的差异。方法：FCA配制后实验组利用超声波细胞粉碎仪乳化20min，对照组直接使用，于大鼠右后足垫皮下注射诱导AA后，采用关节炎评分法对关节肿胀程度进行评估，作X线照片后记录放射学指数（RI），并用透射电镜观察眉窝淋巴结和病变关节滑膜的超微结构的变化，比较两组大鼠各项指标的差异。结果：实验组大鼠的关节肿胀先于对照组发生，程度要低；透射电镜显示实验组呈典型的类风湿性关节炎病变；两组大鼠关节的放射学指数无显著差异。结论：两种方法均能成功诱导大鼠产生佐利性关节炎。利用超声波细胞粉碎仪乳化后的FCA更容易被大鼠吸收，造模周期短。病理组织更典型，成功率更高。为研究类风湿性关节炎（rheumatoid arithritis，RA）的发病机制及药物疗效提供理想的实验模型。

Ultrasonic Cell Smash Instrument and Making Model of Adjuvant Arthritis

Objective： To compare the difference of rat adjuvant arthritis （AA） induced by Freund＇s complete adjuvant（FCA） with the emulsion of ultrasonic cell smash and that by simple FCA. Method： After the preparation of FCA, use the uhrasonic cell was smashed to cmulsificate for 20min for the experimental group. The control group was introduced FCA directly by injecting FCA on the right foot pad of rats to induce AA. Then we used arthritis index to assess the joint swelling , re corded radiology index （RI） after X-ray photographs, and observed the uhrastructural changes eyebrow waterloo lymph nodes and synovial lesions by means of transmission and compared the differences of various indicators. Results： The joint swelling of the experimental group occurred earlier than that of the control group, and the extent was lower. Transmission electron microscopy revealed that the experimental group was typical rheumatoid arthritis disease; there was no significant difference on the joint radiology index between the two groups. Conclusion： Both methods could successfully induced rat adjuvant arthritis. FCA with the emulsification of ultrasonic cell smash was more likely to be absorbed in rats; the modeling cycle is shorter; the pathological organizations were more typical; the success rate was higher ,which made it an ideal experimental model for the study of pathogenesis of rheumatoid arthritis （rheumatoid arithritis, RA） and drug efficacy.