瘦素受体基因Gln223Arg多态性与温州地区高血压合并肥胖的相关性研究

目的 了解温州地区汉族人群瘦素受体Gln223Arg基因型分布;探讨该基因多态性与高血压合并肥胖的关系.方法 运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法 测定无亲缘关系的321例温州地区汉族人群的瘦素受体基因Gln223Arg多态性(对照组111例,高血压病合并肥胖组106例,高血压病非肥胖组104例).结果 高血压病合并肥胖组瘦素受体基因Gln223Arg的GG、GA和AA 3种基因型的表达频率分别为0.604、0.377和0.019,G和A等位基因频率分别为0.769和0.231;高血压病非肥胖组3种基因型的表达频率分别为0.712、0.279和0.010,G和A等位基因频率分别为0.865和0.135;对照组3种基因型的表达频率分别为0.802、0.189和0.009,G和A等位基因频率分别为0.896和0.104;Gln223Arg位点多态性的分布频率在对照组和病例组间差异有统计学意义(x2=6.310,P<0.05),且A等位基因频率在高血压合并肥胖患者中显著高于非肥胖组(x2=6.532,P<0.05).结论 瘦素受体基因Gln223Arg多态性与温州地区汉族人群高血压合并肥胖相关.

Study on the relationship between Gln223Arg variation in leptin receptor gene and hypertension complicated with obesity in Wenzhou population

Objective To study the genotype distribution of Gln223Arg in Han population in Wenzhou area,and discuss its association with hypertension patients with obesity.Methods The genotypes of Gln223Arg variant in leptin receptor gene were determined by polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP) assay in 321 unrelated subjects of Wenzhou Han population,including 111 normal subjects(the control group),106 cases of hypertension patients with obesity and 104 cases of hypertension pat...