食管癌细胞膜提取物体外诱导胚胎胸腺细胞抗癌效应的研究

本文探讨增强胚胎胸腺细胞抗肿瘤效应的方法。采用食管癌细胞膜抗原体外诱导培养的胚胎胸腺细胞 ;用3 H TdR掺入法检测其对食管癌细胞的细胞毒效应 ;用SP 免疫组化法检测其经诱导后细胞表面抗原的变化。结果经食管癌细胞膜抗原诱导 3～ 9d的胚胎胸腺细胞对食管癌细胞的杀伤率明显大于未经诱导的胚胎胸腺细胞 ;胚胎胸腺细胞经诱导后CD4 + /CD8+ 细胞无明显变化 ,但诱导后出现少量CD5 6细胞。食管癌细胞膜抗原体外诱导胚胎胸腺细胞可增强其对食管癌细胞的细胞毒作用

Study on the Induction of Anti-cancer Effect of Embryonic Thymocytes by Esophageal Carcinoma Membrane Antigen in Vitro

To find out the method to promote the anti cancer effect of embryonic thymocytes,the thymocytes were cultured with cell membrane antigens from esophageal carcinoma The cytotoxicity of esophageal cancer cells by thymocytes was determined through 3 H TdR incorporation,and the changes of cell surface antigens after induction were observed by SP immunocytochemical assay Experimental results showed that the cytotoxic effect of embryonic thymocytes on the esophageal cancer cells induced by the esophageal carcinoma membrane antigens 3～9 days after the induction was greater than those of the uninduced embryonic thymocytes There was no significant change on the CD4 +/CD8 + cells,but small amount of CD56 + cells appeared after induction It concludes that the anti cancer effect of embryonic thymocytes could be enhanced by the induction in vitro with the cell membrane antigen from carcinoma of esophagus,suggesting that this method of induction is of value in the treatment of esophageal cancer