感染旋毛虫小鼠外周血T淋巴细胞及其亚群和血清...

Changes in T lymphocytes and their subpopulations and IgG in peripheral blood from mice infected with Trichinella spiralis]

Acid alpha-naphthyl esterase (ANAE), a cytoplasmic marker, was used to identify the T lymphocytes and their subpopulations in peripheral blood of mice infected with Trichinella spiralis, and Dot-ELISA was used to identify the serum IgG antibody of the infected mice. The results showed that T lymphocytes increased on the d3 after infection, reaching the peak on d14, and then remained in number greater than normal up to d77. The spotted granular ANAE positive cells (Help T cells, Th) decreased and the scattered granular ANAE positive cells (Suppressor T cells, Ts) increased, leading to a drop of the Th/Ts ratio. The reduction in host immune function during T. spiralis infection might be related to the drop of Th/Ts ratio. At the same time, the serum IgG antibody increased on d7 after infection, reaching the peak on d28, and remained higher up to d140. The results indicated that the host's cellular immunological level was lower while the host's IgG antibody level was higher during T. spiralis infection.