| 多噬棘阿米巴电泳核型初报 |
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| 引用本文： | 刘家英. 多噬棘阿米巴电泳核型初报[J]. 中国寄生虫学与寄生虫病杂志, 1992, 10(1): 43-44 |
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| 作者姓名： | 刘家英 |
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| 作者单位： | 华东师范大学生物学系 上海 |
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| 摘 要： | 应用脉冲电场凝胶电泳(PFGE)方法,对一种致病性自由生活阿米巴——多噬棘阿米巴的染色体DNA大分子进行分离,并对该寄生原虫的电泳核型进行了观察。所得到的初步结果显示,该虫株的染色体数目在10条以上。在电泳凝胶上可分辨的最大染色体接近2mb,最小的染色体在200kb左右。
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| 关 键 词： | 多噬棘阿米巴 脉冲电场凝胶电泳 电泳核型 |
ON ELECTROPHORETIC KARYOTYPE OF ACANTHAMOEBA POLYPHAGA |
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| Liu Jia-Ying. ON ELECTROPHORETIC KARYOTYPE OF ACANTHAMOEBA POLYPHAGA[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1992, 10(1): 43-44 |
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| Authors: | Liu Jia-Ying |
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| Affiliation: | Department of Biology, East China Normal University, Shanghai. |
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| Abstract: | An electrophoretic karyotype of Acanthamoeba polyphaga has been preliminarily analysed by means of pulse field gel electrophoresis (PFGE).Ten chromosomal DNA bands are distinguishable on the gel.Using yeast (Saccharomyces cerevisiae) chromosomal DNA as size standard,we estimate the size of the chromosomes to be between about 200 kilobase pairs (kb) and 2 megabase pairs (mb). |
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| Keywords: | Acanthamoeba polyphaga pulse field gel electrophoresis electrophoretic katyotype. |
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