| DNA探针用于中华按蚊和嗜人按蚊的分类研究 |
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| 引用本文： | 牛玲玲 袁征. DNA探针用于中华按蚊和嗜人按蚊的分类研究[J]. 中国寄生虫学与寄生虫病杂志, 1992, 10(4): 267-270 |
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| 作者姓名： | 牛玲玲 袁征 |
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| 作者单位： | 中国预防医学科学院寄生虫病研究所，中国预防医学科学院寄生虫病研究所，中国预防医学科学院寄生虫病研究所，School of Biological Sciences，Queen Mary College 世界卫生组织疟疾、血吸虫病和丝虫病合作中心上海 200025，世界卫生组织疟疾、血吸虫病和丝虫病合作中心上海 200025，世界卫生组织疟疾、血吸虫病和丝虫病合作中心上海 200025 |
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| 摘 要： | 本项研究首次报道了我国中华按蚊和嗜人按蚊DNA基因库的建立,并从中华按蚊DNA基因库中筛选出一特异的DNA片段,以此为DNA探针,分别与中华按蚊DNA和嗜人按蚊DNA进行斑点杂交试验,其结果证明该DNA探针可与任何发育期的中华按蚊DNA杂交而不与嗜人按蚊DNA杂交,该探针敏感性高,可检测出7.5ng的中华按蚊DNA,大约相当于1个蚊虫总DNA的1/150,可用于区别中华按蚊和嗜人按蚊。
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| 关 键 词： | 中华按蚊 嗜人按蚊 DNA探针 基因库 |
DNA PROBE FOR IDENTIFICATION OF SIBLING SPECIES OF ANOPHELES SINENSIS AND ANOPHELES ANTHROPOPHAGUS |
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| Niu Ling-Ling,Yuan Zheng,Coi Gao-YingInstitute of Parasitic Diseases,Chinese Academy of Preventive Medicine,Shanghai C. A. Molcolm School of Biological Sciences,Queen Mary College. DNA PROBE FOR IDENTIFICATION OF SIBLING SPECIES OF ANOPHELES SINENSIS AND ANOPHELES ANTHROPOPHAGUS[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1992, 10(4): 267-270 |
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| Authors: | Niu Ling-Ling Yuan Zheng Coi Gao-YingInstitute of Parasitic Diseases Chinese Academy of Preventive Medicine Shanghai C. A. Molcolm School of Biological Sciences Queen Mary College |
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| Affiliation: | Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai. |
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| Abstract: | Genomic DNA libraries of Anopheles sinensis and Anopheles anthropophagus were constructed.The positive clones suitable for discrimination sibling species of An.sinensis and An.anthropophagus were screened and a clone from An.sinensis DNA library was ceceted and the insert DNA was used as a DNA probe to test dot blot of genomic DNA from An.sinensis and An.anthropophagus.The results showed that the DNA probe hybridized with all stages of An.sinensis DNA,but had very weak hybridization signal with An.anthropophagus DNA.The probe was very sensitive and could delect as little as 7.5ng An.sinensis which represents approximately one-150th part of the total DNA from single mosquito.The results demonstrated that the DNA probe reported here could be used to distinguish species of An.sinensis from An.anthropophagus. |
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| Keywords: | Anopheles sinenses Anopheles anthropophagus gcnomic DNA DNA probe |
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