| 血吸虫肝卵免疫酶试验切片载体的改进 |
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| 引用本文： | 顾启章. 血吸虫肝卵免疫酶试验切片载体的改进[J]. 中国寄生虫学与寄生虫病杂志, 1992, 10(1): 59-61 |
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| 作者姓名： | 顾启章 |
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| 作者单位： | 浙江医科大学杭州分校寄生虫学教研室，浙江医科大学杭州分校组织胚胎学教研室，浙江医科大学杭州分校组织胚胎学教研室 杭州 310003，杭州 310003，杭州 310003 |
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| 摘 要： | 应用PVC薄膜代替载玻片作肝卵组织切片载体,进行免疫酶染色试验(简称PVC-IEST)。与玻片法免疫酶染色试验(IEST)比较,两法同时检测78份血吸虫病患者血清,结果PVC-IEST和IEST阳性率分别为96.2％和97.4％。此外,用PVC-IEST检测粪便孵化阳性的血吸虫病患者血清164份和粪便孵化阴性而COPT阳性的血清15份,其PVC-IEST阳性率为97.2％(174/179)。检测6份并殖吸虫病患者血清及并殖吸虫病犬血清6份,均为阴性。健康人血清63份,62份阴性,仅1例假阳性。
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| 关 键 词： | 免疫酶染色 血吸虫病 日本血吸虫 血清学 |
AN IMPROVEMENT OF LIVER EGG-IMMUNOENZYMATIC STAINING TECHNIQUE FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA |
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| Gu Qi-Zhang,He Jia-Gan,Chen He Hangzhou Branch,Zhejiang Medical University,Hangzhou. AN IMPROVEMENT OF LIVER EGG-IMMUNOENZYMATIC STAINING TECHNIQUE FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1992, 10(1): 59-61 |
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| Authors: | Gu Qi-Zhang He Jia-Gan Chen He Hangzhou Branch Zhejiang Medical University Hangzhou |
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| Affiliation: | Hangzhou Branch, Zhejiang Medical University. |
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| Abstract: | Using PVC membrane instead of common slide in liver egg-immunoenzymatic staining technique (IEST) for diagnosis of schistosomiasis japonica to detect specific antibodies in sera from 179 cases with schistosomiasis japonica,the positive rate was 97.2% (174/ 179).Sera from 63 healthy individuals revealed in one positive (1.5%).No cross reaction was observed in sera from 6 cases of paragonimiasis.PVC-IEST and SLIDE-IEST were comparatively performed on sera from 78 cases with schistosomiasis japonica,the positive rates were 96.2% (75/78) and 97.4% (76/78),respectively (P>0.05).As PVC-IEST is simpler to perform than SLIDE-IEST,it is suggested that the former is more suitable for application in the field for diagnostic purposes. |
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| Keywords: | Immunoenzymatic staining schistosomiasis Schistosoma japonicum serology. |
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