日本血吸虫病宿主肿瘤坏死因子活性的研究

采用L929细胞杀伤法,对晚期日本血吸虫病(晚血)患者和感染日本血吸虫尾蚴8wk、16wk及杀虫后8wk的新西兰兔,进行了外周血单核细胞(PBMC)的肿瘤坏死因子(TNF)诱生能力和血清TNF活性测定。晚血患者PBMC的TNF诱生能力明显低于正常人,而血清中TNF活性明显增高(P<0.01)。感染8wk和16wk兔PBMC的TNF诱生能力较正常兔显著增高,尤以感染8wk时为甚,血清TNF活性亦相应增高(P<0.01);在杀虫治疗后8wk,其PBMC的TNF诱生能力和血清TNF活性均降至正常。晚血患者血清TNF活性的增高可能参与肝纤维化过程。

THE ACTIVITY OF TUMOR NECROSIS FACTOR IN SCHISTOSOMIASIS JAPONICA

The activity of tumor necrosis factor (TNF) in both the culture supernatant of pheripheral blood mononuclear cells (PBMC) and the serum was examined in 20 patients of advanced schisto-somiasis at 8th week after pyquiton treatment,and 6 rabbits infected with S.japonicum cercariae at 8th and 16th weeks post-infection and using L929 cell cytotoxic assay.The results showed that the capacity of PBMC to produce TNF in response to LPS from patients with late schistosomiasis was significantly lower than that from normal subjects (P<0.01),whereas the activity of TNF in serum was significantly elevated (P<0.01).The capacity of PBMC to produce TNF and the TNF activity in serum of infected rabbits showed sharp rise at 8th week post-infection (P<0.01),and remained high at 16th week,then returned to normal level 8 weeks after treatment.The persistent rise of TNF in the circulation indicate that TNF might take part in the formation of hepatic fibrosis.