子宫内膜癌患者T细胞亚群、红细胞免疫与Ag-NORs定量分析的临床意义

目的 探讨子宫内膜癌患者的免疫功能变化及临床意义，方法 采用单隆抗体直接免疫荧光染色法，红细胞花环试验及KL型肿免疫图象分析仪，对21例子宫内膜癌患者和20名正常对照组分别测定其外周血T细胞亚群，红细胞免疫C3b受体花环率（E－C3bRR%)与红细胞循环免疫复合物花环率（ICR％），核仁银染区面积／细胞核面积比值（I．S％），结果 子宫内膜癌患者CD3^ T细胞，CD4 ^ T细胞，CD4^ ./CD8^ 比值，E－C3 bRR%,I.S%值明显降低；CD8^ T细胞，T－ICR％明显升高，与对照组相比较差异显著（P＜0．01），结论 子宫内膜癌患者免疫功能低下，联合检测T细胞亚群，红细胞免疫与Ag-NORs可较全面反映子宫内膜癌患者的免疫功能状态，并可指导临床治疗。

Significance of T lymphocyte subset, erythrocyte immune function and Ag-NORs in nucleolar organizer regions in patients with carcinoma of endometrium

AIM To study the significance of T lymphocyte subset erythrocyte immune function and argyrophilic protein in nucleolar organizer regions (Ag NORs) in patients with carcinoma of the endometrium. METHODS CD + 3 T cell, CD + 4 T cell, CD + 8 T cell, CD + 4/CD + 8 were determined by flow cytometery; E C 3 bRR% and E ICR% were assayed by E C 3 bRR and ICR. Granules area of Ag NORs/nuclear area (I.S%) were studied by KL image analysis system. RESULTS CD + 3 T cell, CD + 4 T cell, CD + 4/CD + 8, E C 3 bRR%, I.S% in the patients were significantly lower than those in the healthy group ( P <0.01), however, the values of E ICR%, CD + 8 T cell were significantly higher than those of control group ( P <0.01). CONCLUSION Immune function decreases in the patient. The detection of T lymphocyte subset, erythrocyte function and Ag NORs can express the immunologic function of carcinoma of the endometrium, and so is helpful for directing clinical therapy.