基于HPLC指纹图谱不同产地板蓝根主要活性成分与其根际土壤理化性质的相关性研究

目的 分析不同产地板蓝根Isatidis Radix主要活性成分与其根际土壤理化性质的相关性，探究土壤理化性质对板蓝根质量的影响。方法 采用HPLC建立板蓝根的指纹图谱，测定板蓝根中胞苷、尿苷、腺苷、鸟苷和(R, S)-告依春5个指标成分的含量；应用Origin软件对板蓝根主要指标成分与其根际土壤理化性质进行相关性分析。结果 建立了16批板蓝根的HPLC指纹图谱，指认了5个成分，指纹图谱相似度为0.858～0.998，具有较好的相似性；含量测定结果表明，不同产地板蓝根药材中5个指标成分含量存在较大差异；板蓝根主要活性成分胞苷含量与土壤有效磷含量呈显著正相关（P<0.05），尿苷含量与pH呈显著负相关（P<0.05），腺苷含量与含水量和速效钾含量呈显著正相关（P<0.05）。结论 板蓝根主要活性成分含量与其根际土壤理化性质具有一定的相关性，土壤pH、含水量、有效磷、速效钾是影响板蓝根药材质量的主控因子。为进一步探讨板蓝根适宜的土壤环境和提升其质量标准提供一定依据，为板蓝根药材的标准化种植与质量控制提供参考。

Correlation between main active components of Isatidis Radix from different producing areas and physicochemical properties of its rhizosphere soil based on HPLC fingerprint

Objective To analyze the correlation between the main active components of Banlangen (Isatidis Radix) from different producing areas and the physicochemical properties of its rhizosphere soil, and to explore the effect of soil physicochemical properties on the quality of Isatidis Radix. Methods The fingerprint of Isatidis Radix was established by HPLC, and the contents of cytidine, uridine, adenosine, guanosine and (R, S)- goitrin in Isatidis Radix were determined. The correlation between the main index components of Isatidis Radix and the physicochemical properties of its rhizosphere soil was analyzed by Origin software. Results The HPLC fingerprints of 16 batches of Isatidis Radix were established, and 5 components were identified. The similarity of fingerprint ranged from 0.858 to 0.998, which showed good similarity. The results of content determination showed that there were significant differences in the contents of five index components in Isatidis Radix from different producing areas. The content of cytidine, the main active component of Isatidis Radix, was significantly positively correlated with the content of available phosphorus in soil (P < 0.05). The content of uridine was significantly negatively correlated with pH (P < 0.05). The content of adenosine was significantly positively correlated with soil moisture content and available potassium content (P < 0.05). Conclusion The content of main active components in Isatidis Radix has a certain correlation with the physical and chemical properties of its rhizosphere soil. Soil pH, soil moisture content, available phosphorus and available potassium are the main controlling factors affecting the quality of Isatidis Radix. This study provides a basis for further exploring the suitable soil environment and improving the quality standard of Isatidis Radix, and provides a reference for the standardized cultivation and quality control of Isatidis Radix.