| 脂多糖对B细胞的活化作用及机制的初步研究 |
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| 引用本文： | 钱莉,佟大可,潘兴元,田芳,龚卫娟,季明春.脂多糖对B细胞的活化作用及机制的初步研究[J].第二军医大学学报,2011,32(11):1231-1234. |
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| 作者姓名： | 钱莉 佟大可 潘兴元 田芳 龚卫娟 季明春 |
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| 作者单位： | 1. 扬州大学医学院病原生物学与免疫学教研室,扬州,225001
2. 第二军医大学长海医院骨科,上海,200433 |
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| 基金项目： | 国家自然科学基金(81001308), 江苏省自然科学基金(BK2010315). |
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| 摘 要： | 目的观察TLR4配体脂多糖(LPS)对B细胞功能影响及相关的信号转导通路。方法利用免疫磁珠法分选小鼠脾脏CD19+B细胞,体外用LPS刺激后,CBA(cytometric bead array)法检测B细胞分泌的Ig(immunoglobulin)亚型;流式细胞术(flow cytometry,FCM)检测B细胞表型;CBA法检测培养上清中细胞因子IL-6、IL-10、IL-12p70、TNF浓度。利用ERK、JNK、p38MAPK和NF-κB信号转导抑制剂检测B细胞内细胞因子分泌的信号转导通路。结果 LPS可以诱导B细胞产生IgG1-κ和IgM-κ抗体,上调B细胞表面CD40、CD80、CD86和MHCⅡ类分子的表达,促进IL-6、IL-10和TNF的高分泌。JNK、p38MAPK和NF-κB信号转导通路调控B细胞IL-6、IL-10和TNF的分泌。结论 LPS可以通过诱导抗体产生、上调共刺激分子表达和促进细胞因子分泌等多方面调节B细胞功能。
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| 关 键 词： | 脂多糖类 B淋巴细胞 功能 信号转导 |
| 收稿时间： | 2011/3/17 0:00:00 |
| 修稿时间： | 2011/8/21 0:00:00 |
Lipopolysaccharide activates B cells and the underlying mechanisms |
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| QIAN Li,TONG Da-ke,PAN Xing-yuan,TIAN Fang,GONG Wei-juan and JI Ming-chun.Lipopolysaccharide activates B cells and the underlying mechanisms[J].Academic Journal of Second Military Medical University,2011,32(11):1231-1234. |
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| Authors: | QIAN Li TONG Da-ke PAN Xing-yuan TIAN Fang GONG Wei-juan and JI Ming-chun |
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| Institution: | 1. Department of Etiology and Immunology, Medical School of Yangzhou University, Yangzhou 225001, Jiangsu, China;2. Department of Othorpeadics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;1. Department of Etiology and Immunology, Medical School of Yangzhou University, Yangzhou 225001, Jiangsu, China;2. Department of Othorpeadics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;1. Department of Etiology and Immunology, Medical School of Yangzhou University, Yangzhou 225001, Jiangsu, China;2. Department of Othorpeadics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;1. Department of Etiology and Immunology, Medical School of Yangzhou University, Yangzhou 225001, Jiangsu, China;2. Department of Othorpeadics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;1. Department of Etiology and Immunology, Medical School of Yangzhou University, Yangzhou 225001, Jiangsu, China;2. Department of Othorpeadics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;1. Department of Etiology and Immunology, Medical School of Yangzhou University, Yangzhou 225001, Jiangsu, China;2. Department of Othorpeadics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | Objective To observe the effects of lipopolysaccharide(LPS) on B cell function and the related signal transduction pathways.Methods Freshly purified splenic B cells(CD19+ B) of mice were isolated by magnetic activated cell sorting.After stimulation with LPS,the culture supernatants were collected for immunoglobulin isotyping by cytometric bead array(CBA) technology.Flow cytometry was used to analyze costimulatory molecule expression and culture supernatants were also collected for measuring IL-6,IL-10,IL-12... |
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| Keywords: | lipopolysaccharides B-lymphocytes function signal transduction |
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