调控CIAPIN1基因表达对食管癌细胞生长及侵袭性的影响

目的 采用重组慢病毒CIAPIN1表达载体和CIAPIN1沉默载体感染食管癌EC9706细胞,观察调控CIAPIN1基因表达对细胞增殖、细胞周期、细胞凋亡及体外侵袭转移的影响.方法 构建CIAPIN1表达慢病毒载体和CIAPIN1 siRNA慢病毒载体,包装得到重组慢病毒；分别感染食管癌EC9706细胞,筛选得到稳定C...

Effects of CIAPIN1 gene expression on growth and invasion behavior of esophageal cancer cells

Aim To Investigate the effect of CIAPIN1 expression on proliferation, cell cycles, apoptosis, in vivo invasion and migration of EC9706, a kind of esophageal cancer cell line, by transfection of recombinant lentiviral vectors for expression and silencing of CIAPIN1 rspectively. Methods EC9706 cells were transfected with CIAPIN1 expression vector and CIAPIN1 siRNA vector respectively, and cell lines with stable over-expression and low expression of CIPAN1 were obtained. RT-PCR and western-blot, MTT, flow cytometry method and transwell were used to study the expression of CIAPIN1, cell proliferation, cell cycles and apoptosis, and cells invasion and migration respectively. Results CIAPIN1 group, expressed inhibited growth of EC9706 cells, decreased cells in S and G2/M phase, increaed cells in G0/G1 phase, elevated apoptosis and reduced cells passing through matrigel compared with control groups. Conclusion Lentiviral vector-mediated over-epression of CIPAIN1 could inhibit the proliferation, promote apoptosis, and reduce invasion and migration of EC9706, a kind of eophageal cancer cells.