| 应用MS32单位点小卫星DNA探针检测小儿急性淋巴细胞性白血病PCR－DNA指纹 |
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| 引用本文： | 李玉新,杨贵贞.应用MS32单位点小卫星DNA探针检测小儿急性淋巴细胞性白血病PCR－DNA指纹[J].中国免疫学杂志,1994(2). |
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| 作者姓名： | 李玉新 杨贵贞 |
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| 作者单位： | 白求恩医科大学免疫学教研室 |
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| 摘 要： | 利用单位点ＰＣＲ－ＤＮＡ指纹，检测小儿急性淋巴细胞性白血病患者外周血淋巴细胞（混有白血病细胞）和粒细胞基因组ＤＮＡ中ＤＩＳ８小卫星位点上ＶＮＴＲ，并将两者进行比较，发现混有白血病细胞的淋巴细胞ＰＣＲ－ＤＮＡ指纹图中存在带型的增多和带强度的变化，表明在此位点白血病细胞的ＶＮＴＲ发生了改变。
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| 关 键 词： | PCR－DNA指纹，小儿急性淋巴细胞性白血病，D1S8小卫星位点 |
DETECTION OF PCR-DNA FINGERPRINTING OF CHILDRENWITH ACUTE
LYMPHOBLASTIC LEUKEMIA BY MS32 SINGLELOCUS MINISATELLITE DNA
PROBE |
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| Li Yuxin,Yang Guizhen.DETECTION OF PCR-DNA FINGERPRINTING OF CHILDRENWITH ACUTE
LYMPHOBLASTIC LEUKEMIA BY MS32 SINGLELOCUS MINISATELLITE DNA
PROBE[J].Chinese Journal of Immunology,1994(2). |
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| Authors: | Li Yuxin Yang Guizhen |
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| Institution: | Li Yuxin,Yang Guizhen. Department of Immunology,Norman
Bethune Univer of Medical Science,Changchun 130021 |
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| Abstract: | etection
and comparison of VNTR at DIS8 minisatellite locus in genomic DNA
oflymphocytes and granulocytes from the same case of children acute
lymphoblastic leukemia-using PCR-DNA fingerprinting were carried out.
The novel bands and shifts in band intensi-ties in PCR-DNA
fingerprinting of lymghocytes mixed with leukemic cells were
discovered. That indicates the alteration of VNTR of leukemic cells. |
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| Keywords: | PCR-DNA fingerprinting Children acute lymphoblastic leukemia DIS8 minisatellite locus |
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