多孔磷酸钙陶瓷在动态SBF中类骨磷灰石形成的研究

本实验在模拟体液（SBF）以静止、等于或大于骨骼肌组织内体液正常生理流率（2ml/100ml.min)的流动条件下，研究多孔磷酸钙陶瓷上类骨磷灰石的形成，结果表明，SBF在生理流率（2ml/100ml.min)情况下，材料仅在多孔磷酸钙陶瓷孔隙内部形成类骨磷灰石，静态（0ml/100ml.min)情况下，多孔磷酸钙陶瓷材料表面有类骨磷灰石形成；高于生理流率（10ml/100ml/min)时，表面和断面均无类骨磷灰石形成，但如果增加SBF中的Ca^2 ,HPO4^2-的浓度，则在孔隙内部有类骨磷灰石形成，生理流条件下的结果与多数肌肉内植入磷酸钙陶瓷试验的结果一致。仅在多孔材料内部成骨，这个结果表明，比起通常使用的静态浸泡试验，SBF以生理流率流动的动态体外试验能够更好地模拟类骨 磷灰石生长的体内环境，动态SBF对了解类骨磷灰石形成，进而了解磷酸钙陶瓷在体内诱导成骨机理是十分有用的。

A Study of Bone-like Apatite Formation on Porous Calcium Phosphate Ceramics in Dynamic SBF

This study aimed at investigating the influence of the flow rate of simulated body fluid (SBF) (2 ml/100 ml.min) of body fluid in skeletal muscle upon the formation of bone-like apatite on porous calcium phosphate ceramics. The in vitro immersion experiment in SBF flowing at normal physiological rate is referred to as dynamic SBF. The results showed that bone-like apatite could only formed in the pores of porous calcium phosphate when SBF flow at physiological rate (2 ml/100 ml.min) of body fluid in skeletal muscle. At the same time, bone-like apatite could form both in the pores and on the surface of the samples if the flowing physiological solution is 1.5 SBF. When the flowing speed of SBF is higher than normal physiological speed (10 ml/100 ml.min), no bone-like apatite could be detected both on the surface and in the pores of the materials. This result is in concordance with animal experiments. The dynamic SBF simulates the biological environment of bone-like apatite formation in body better than static SBF (SBF does not flow). This method is very useful for the research of the mechanism of bonelike apatite formation, which is the key step of bone growth on biomaterials, and can be used as an effective approach to investigate mechanism of the osteoinduction of calcium phosphate in nonosseous tissues in vivo.